蚯蚓纤溶酶化学修饰的研究  

Studies on Chemical Modification of Fibrinolytic Enzyme from Pheretima

作　　者：莫宏波[1] 伍晓斌[2] 徐凤彩[3] 

机构地区：[1]暨南大学医学院,广东广州510632 [2]中国科学院微生物研究所,北京100080 [3]华南农业大学生命科学院,广东广州510642

出　　处：《药物生物技术》2004年第5期306-310,共5页Pharmaceutical Biotechnology

基　　金：广东省高教厅基金(200001)

摘　　要：以三聚氰氯为活化剂,采用活化聚乙二醇(PEG)对环毛蚯蚓纤溶酶进行化学修饰,优化了修饰条件,得到了PEGEFE的加合物(修饰酶),并研究了PEGEFE的部分酶学性质。修饰酶的活力为天然酶的85%,结果表明酶的活性中心基本保持不变。对修饰酶的残留氨基测定表明,70%的可滴定氨基参加了反应,且EFE的活性改变较小,可以推测PEG是连接在蛋白质表面上的。EFE was separated from Pheretima and modified with activated PEG. The optimum conditions for chemical modification were selected. The activity of modified EFE is 85% of that of free EFE. It means that the activity site of EFE almost weren't changed. The modification rate of amino residues is 70%. We could guess that activated PEG connected with the surface of EFE protein. It showed the modified EFE could improve its thermal and pH stability.

关 键 词：修饰酶 改变 PEG 化学修饰 天然 活性中心 氨基 蚯蚓纤溶酶 酶学性质 蛋白质 

分 类 号：R978.1[医药卫生—药品] Q55[医药卫生—药学]