蛋白激酶C表达上调与KBV200细胞多药耐药的关系  

Up-regulation of PKC Expression and Multidrug Resistance in KBV200 Cells

作　　者：孙爱民[1] 袁亚维[1] 李传刚[2] 陈龙华[1] 

机构地区：[1]第一军医大学南方医院放疗科,广州市510515 [2]第一军医大学南方医院超声诊断科,广州市510515

出　　处：《中国肿瘤临床》2004年第20期1141-1143,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:39870809)

摘　　要：目的:探讨蛋白激酶C(PKC)的表达上调与肿瘤多药耐药的关系。方法:32P掺入法测定多药耐药KBV200细胞株的PKC活性;Westernbloteing法检测PKC亚型表达和亚细胞分布;MTT法检测细胞耐药性;实验组应用200nmol/L佛波酯(PMA)预孵育KBV200细胞。结果:PMA可提高KBV200细胞的PKC总活性和膜组分PKC活性,降低浆组分PKC活性(P<0.01);使膜组分PKCα表达增加,浆组分PKCα表达降低,膜组分PKCβ无明显变化,浆组分PKCβ的表达稍增强;PMA可升高长春新碱(VCR)、阿霉素(ADR)对KBV200细胞的IC50值(P<0.01)。结论:PMA使KBV200细胞耐药性增加,可能与PKC表达上调有关。Objective: To investigate the relationship between protein kinase C(PKC) and multidrug resistance(MDR) in KBV200 cells. Methods: PKC activity was assayed by the measurement of the incorporation of 32P from ATP into peptide substrates. Western blotting was used for the assessment of PKC isoform expression. PKC activitor PMA were used to preincubate KBV200 cells to evaluate the importance of PKC in MDR. The cell inhibitory rate was evaluated by MTT. Results: The PKC activity of total and membrane fraction were higher in test group than those in control group, while PKC activity of cytosol fraction was lower. PKCα expression was upregulated in membrane fraction and downregulated in cytosol fraction in KBV200 cells after PMA preincubation. PKCβ expression was higher slightly in cytosol fraction but no alteration in membrane fraction in test group compared with control group. The values of IC50 of VCR and ADR in test group were increased to 2275.5nmol/L and 233.25nmol/L respectively(P<0.01). Conclusion: PMA can increase the multidrug resistance of KBV200 cells, suggesting that PKC may be involved in the mechanism of multidrug resistance of tumor cells.

关 键 词：蛋白激酶C 多药耐药 长春新碱 阿霉素 佛波酯 

分 类 号：R73-3[医药卫生—肿瘤]