紫外分光光度法同时测定厚朴酚与和厚朴酚的含量及活性研究  

SIMULTANEOUS DETERMINATION OF MAGNOLOL AND HONOKIOL BY UV SPECTROPHOTOMETRY AND STUDY ON FREE RADICAL SCAVENGER ACTIVITY

作　　者：保志娟[1] 杨雪琼[1] 丁中涛[1] 曹秋娥[1] 邹永明[1] 

机构地区：[1]云南大学化学系,昆明650091

出　　处：《天然产物研究与开发》2004年第5期435-438,共4页Natural Product Research and Development

基　　金：国家自然科学基金 ( 3 0 2 60 0 14 ) ;云南省中青年学术基金;云南省技术带头人后备人才培养基金 (YP0 0 2 4)

摘　　要：使用pH =8.93的KH2 PO4 NaOH缓冲液 (0 .0 2 5mol/L) ,根据混合物含量测定的原理 ,在 2 89.4nm和 316 .4nm处测定溶液吸光度来测定厚朴酚与和厚朴酚的含量 ,在 5 17nm处考察厚朴酚与和厚朴酚清除DPPH·的活性 ,建立了同时测定厚朴酚与和厚朴酚含量的紫外分光光度法 ,初步考察了二者的自由基清除活性。DPPH·可作为二者抗氧化活性的研究方法。In order to establish a UV spectrophotometric method for simultaneous determination of magnolol and honokiol,and study their free radical scavenger activity preliminarily.In the KH 2PO 4-NaOH buffer(0.025 mol/L,pH=8.93),magnolol and honokiol were determined by UV spectrophotometry at 289.4 nm and 316.4 nm.The scavenger activities were assayed at 517 nm by UV spectrophotometry.This method can determine magnolol and honokiol for some traditional Chinese medicine simply and quickly,and their free radical scavenger activities can be determined by DPPH·method.

关 键 词：和厚朴酚 紫外分光光度法 含量测定 DPPH 自由基清除 同时测定 活性研究 KH2PO4 酚含量 使用 

分 类 号：Q523[生物学—生物化学] R286.0[医药卫生—中药学]