正常骨和骨肿瘤组织雌激素受体分析  

Analysis of Estrogen Receptors in Normal Bone and Bone Tumor Tissues

作　　者：韩守威[1] 何斌[1] 唐茜萍[1] 彭芝兰[1] 郑虎[2] 翁玲玲[2] 

机构地区：[1]华西医科大学附属第二医院妇产科 [2]华西医科大学药学院药物化学教研室

出　　处：《华西医科大学学报》1993年第2期160-162,共3页Journal of West China University of Medical Sciences

基　　金：中日骨质疏松治疗药物研究基金

摘　　要：用葡聚糖—活性炭Scatchard作图法测定15例不同骨恶性肿瘤组织(其中转移性骨肿瘤3例)及13例正常对照骨组织胞浆的雌激素受体(ER)含量和亲和常数。结果显示,ER平均含量正常对照组(15.12±14.68fmol/mg蛋白)高于骨恶性肿瘤组织(8.04±6.71fmol/mg蛋白)(P<0.05)。ER亲和常数正常对照组(39.91±20.13×10^(-11)mol/L)高于骨恶性肿瘤组织(18.46±27.10×10^(-11)mol/L)(P<0.025)。ER阳性率在正常对照组织(23.1％)和恶性骨肿瘤组织(26.7％)无明显差别(P>0.05)。本研究表明,骨组织中胞浆ER含量及亲和力变化可能与肿瘤的发生有关;骨恶性肿瘤组织胞浆受体含量降低,而亲和力增加,提示该组织甾体激素受体系统功能的改变或损害。Thirteen human normalbone and fifteen bone tumor tissues wereassayed for estrogen receptor (ER)by Dex-tran-coated Charcoal method (Scatchardplot). The results showed that the con-centrations of ER in normal bone tissues(15.12±14.68 fmol/mg pro) were higherthan that of bone malignant tumors (8.04±6.71 fmol/mg pro) P<0.05). The bind-ing dissociation constants (Kd) of ER inbone tumors (18.46±27.10×10^(-11)mol/L)was lower than those in normal ones(39.91±20.13×10^(-11)mol/L) (P<0.025).The percentages for positive score of ERin normal and tumor ones (23.1%, 26.7%)were not significantly different (P>0.05).Our study indicated that the variations ofconcentration and affinity of ER in bonerelated to the tumor incidence. That cyto-sol ER content was decreased in malignantbone tumors whereas that of affinityincrease suggests an impairment or changeof an intact receptor mechanism in thistissues.

关 键 词：骨肿瘤 正常骨 雌激素 受体 

分 类 号：R738.102[医药卫生—肿瘤]