中国北方肝细胞癌与乙肝病毒感染的紧密关联性  

Close relation of hepatocellular carcinoma to HBV infection in North China

作　　者：高纪东[1] 邵永孚[1] 许杨[2] 明利华[2] 吴志远[2] 刘国亭[2] 王小红 王文红 冯晓丽 孙宗棠[2] 

机构地区：[1]中国医学科学院肿瘤医院腹部外科,北京市100021 [2]中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室,北京市100021

出　　处：《中华肝胆外科杂志》2004年第6期363-365,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：本课题受国家科技攻关计划课题基金资助( 2 0 0 1BA70 3B0 6)

摘　　要：目的　确立乙肝病毒 (hepatitisBvirus,HBV)及丙肝病毒 (hepatitisCvirus,HCV)感染在中国北方肝细胞癌 (hepatocellularcarcinoma,HCC)发生中的病因学地位。 方法　采用AbbottAxSym系统检测 119例连续系列中国北方地区HCC病例的血清HBV、HCV标志 ,对血清HBV标志阴性病例 ,采用免疫组化、巢式PCR和DNA测序等对HBVX基因进行鉴定。结果　 119例HCC中 ,血清HBsAg阳性率为 82 4 % (98/ 119) ;anti HBc阳性率为 94 1% (112 / 119) ;4例血清HBsAg及anti HBc均阴性病例中 ,3例HBVX基因阳性 ,因而HBV感染的阳性率达 99 2 % (118/ 119)。本组anti HCV阳性率 11 8% (14 / 119) ,且均伴有HBV感染标志。结论　中国北方地区HCC与HBV感染具有紧密关联性 ,是本地区HCC发生的主导病因。Objective To define the importance of HBV and HCV infection in pathogenesis of hepatocellular carcinoma (HCC) in North China. Methods A consecutive series of 119 patients with HCC living in North China who were treated in our hospital were included in this study. Serum HBsAg, anti-HBc and anti-HCV were detected by Abbotts AxSym System. For those patients with negative detection of HBV sero-markers, HBV X gene was analyzed with nested PCR, DNA sequencing and immunohistochemistry for its expression. Results Amongst the 119 patients, 98 (82.4%) were HBsAg sero-positive. When a comprehensive set of HBV markers were detected, the rate of HBV infection in these patients reached the level of 99.2% (118/119). Of the 119 cases, 14 (11.8%) were found to be anti-HCV positive. However, all the anti-HCV positive cases had co-infection of HBV. Conclusions In North China, HBV infection is closely related to pathogenesis of HCC. Meanwhile, HBV infection is the major cause for HCC in this region. HCV is an important auxiliary factor for HCC.

关 键 词：HCC HCV 肝细胞癌 HBV感染 阳性率 血清HBV 阴性 DNA测序 基因 中国北方 

分 类 号：R735.7[医药卫生—肿瘤]