胆汁成核效应蛋白的分离纯化与成核活性比较  被引量：7

rification and comparative nucleating potencies of crystallization-promoters in human bile</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=10763956' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('mbztBHz7BfUA3hoRzWtSLHHa4Gl+v4WW6PC9ocmkuqk=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="mbztBHz7BfUA3hoRzWtSLHHa4Gl+v4WW6PC9ocmkuqk="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000006183140' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%a1%b9%e5%bb%ba%e6%96%8c%5d' title='项建斌[1]'>项建斌[1]</a> <a href='/writer/article.aspx?id=100000003326376' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e8%94%a1%e7%ab%af%5d' title='蔡端[1]'>蔡端[1]</a> <a href='/writer/article.aspx?id=100000006506805' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%bc%a0%e5%bb%b6%e9%be%84%5d' title='张延龄[1]'>张延龄[1]</a> <a href='/writer/article.aspx?id=100000012443864' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%a9%ac%e4%bf%9d%e9%87%91%5d' title='马保金[1]'>马保金[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=868&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e5%a4%8d%e6%97%a6%e5%a4%a7%e5%ad%a6%e9%99%84%e5%b1%9e%e5%8d%8e%e5%b1%b1%e5%8c%bb%e9%99%a2%e5%a4%96%e7%a7%91' title='[1]复旦大学附属华山医院外科,上海市200040'>[1]复旦大学附属华山医院外科,上海市200040</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=91171X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e4%b8%ad%e5%8d%8e%e8%82%9d%e8%83%86%e5%a4%96%e7%a7%91%e6%9d%82%e5%bf%97%5d' title='中华肝胆外科杂志'>《中华肝胆外科杂志》</a>2004年第7期453-456,共4页<em>Chinese Journal of Hepatobiliary Surgery</em></p><p class='fund'><strong>基　　金：</strong>本课题受国家自然科学基金(30070737)卫生部基金(98-2-124)资助</p><p class='abstrack'><strong>摘　　要：</strong>目的 纯化并比较各种成核效应蛋白的活性,筛选最具病理学意义的促成核因子。方法 通过ConA-Sepharose 4B亲和层析和Sephadex G-200、Sephadex G-100分子筛凝胶过滤以及超速离心法从胆固醇性结石病人胆汁中分离提纯70 kDa、200 kDa促成核蛋白以及33.5 kDa泡蛋白,在模拟胆汁体系中观察成核因子促进胆固醇结晶生长、析出过程,比较其成核活性。结果 四种候选的成核效应蛋白中,33.5 kDa泡蛋白是活性最强的促成核因子,成核活性强弱依次为33.5 kDa泡蛋白>200 kDa糖蛋白≈α1-酸性糖蛋白>70 kDa糖蛋白,当浓度为100／μg／ml时,33.5 kDa泡蛋白成核活性指数Ig、Ic、It分别为1.52、1.63、0.57,并随着蛋白浓度的增加,促成核活性亦随之增强(F=678.18,P<0.05),且呈剂量依赖效应。结论 胆汁中存在多种促成核作用的糖蛋白,其中33.5 kDa泡蛋白可能与胆石形成关系最为密切。<em><abstract>jective To immunopurify and examine the comparative activities of the candidate pro-nucleating proteins. Methods The 70 kDa and 200 kDa glycoproteins were isolated by immu-naffinity chromatography using ConA-Sepharose 4B, Sephadex G-200 and Sephadex G-100, and the 33. 5 kDa vesicular protein was purified by ultracentrifugation from cholesterol gallstone bile. Comparative effect of adding crystallization-promoters was detected by cholesterol crystal growth. Results A-mongst the 4 glycoproteins, the 33. 5 vesicular protein showed the strongest promoting effects. The potency of these 4 proteins was in the following order: 33. 5 kDa vesicular protein > 200 kDa glyco-protein ≈α1-acid glycoprotein > 70 kDa glycoprotein. The activity indexes Ig, Ic and It of the 33. 5 kDa vesicular protein was 1. 52, 1.63 and 0. 57 respectively, at the concentration of 100 μg/mlo in model bile, and the pro-nucleating activity was enhanced along with increase in concentration of the protein. Conclusions Several glycoproteins exist in the bile may account for the gallstone formation and the 33. 5 kDa vesicular protein plays an important role in cholesterol crystallization.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/zk/search.aspx?key=K%3d%5b%e8%83%86%e6%b1%81%5d' title='胆汁'>胆汁</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%88%90%e6%a0%b8%e6%95%88%e5%ba%94%e8%9b%8b%e7%99%bd%5d' title='成核效应蛋白'>成核效应蛋白</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%b4%bb%e6%80%a7%5d' title='活性'>活性</a> <a href='/subject/article.aspx?id=9229084' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e4%bf%83%e6%88%90%e6%a0%b8%e5%9b%a0%e5%ad%90%5d' title='促成核因子'>促成核因子</a> <a href='/subject/article.aspx?id=7289352' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%ce%911-%e9%85%b8%e6%80%a7%e7%b3%96%e8%9b%8b%e7%99%bd%5d' title='Α1-酸性糖蛋白'>Α1-酸性糖蛋白</a> <a href='/subject/article.aspx?id=71055' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%83%86%e5%9b%ba%e9%86%87%e7%bb%93%e6%99%b6%5d' title='胆固醇结晶'>胆固醇结晶</a> <a href='/subject/article.aspx?id=4918321' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%bb%93%e7%9f%b3%e7%97%85%5d' title='结石病'>结石病</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%80%a7%e6%af%94%5d' title='性比'>性比</a> <a href='/subject/article.aspx?id=6762206' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%88%86%e7%a6%bb%e7%ba%af%e5%8c%96%5d' title='分离纯化'>分离纯化</a> <a href='/subject/article.aspx?id=88450' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%9b%8b%e7%99%bd%e6%b5%93%e5%ba%a6%5d' title='蛋白浓度'>蛋白浓度</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR575.6' title='R575.6'>R575.6</a>[<a href = '/domain/article.aspx?id=493' >医药卫生—消化系统</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=10763956" name="flashVars"> <embed width="695" height="250" src="../flash/relation.swf" type="application/x-shockwave-flash" quality="high" wmode="opaque" flashvars="dataUrl=/ajax/articlerelativegraph.ashx?id=10763956"> </embed> </object> </div> <div class="article-relate-list"> <div class="relate-list"> <p id="ckwx_br"> </p> <h2 name="ckwx" id="ckwx">参考文献：</h2> <div id="listckwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="2jckwx_br"> </p> <h2 name="2jckwx" id="2jckwx">二级参考文献：</h2> <div id="list2jckwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="ohwx_br"> </p> <h2 name="ohwx" id="ohwx">耦合文献：</h2> <div id="listohwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="yzwx_br"> </p> <h2 name="yzwx" id="yzwx">引证文献：</h2> <div id="listyzwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="2jyzwx_br"> </p> <h2 name="2jyzwx" id="2jyzwx">二级引证文献：</h2> <div id="list2jyzwx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="tbywx_br"> </p> <h2 name="tbywx" id="tbywx">同被引文献：</h2> <div id="listtbywx"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> <div class="relate-list"> <p id="xgqklw_br"> </p> <h2 name="xgqklw" id="xgqklw">相关期刊文献：</h2> <div id="listxgqklw"> <p class='submit'> <img src='/images/loading.gif' width='16' height='16' /> 正在载入数据... </p> </div> </div> </div> </div> <div class="article-about"> <div class="about-box"> <h5 class="name">相关的主题<tt><a class="hide" href="#"></a></tt></h5> <div class="about-list"> </div> </div> <div class="about-box"> <h5 class="name">相关的作者对象<tt><a class="" href="#"></a></tt></h5> <div class="about-list" style="display: none;"> </div> </div> <div class="about-box"> <h5 class="name">相关的机构对象<tt><a class="" href="#"></a></tt></h5> <div class="about-list" style="display: none;"> </div> </div> </div> </div> </div> <script type="text/javascript" src="/scripts/articlerelation.min.js"></script> <script type="text/javascript" src="/scripts/articlerightrelation.min.js"></script> <script type="text/javascript" src="/scripts/articledown.min.js"></script> <div class="layer-box download" id="downloadlayer" style="display: none"> <div class="close"> <a onclick="closedown()" href="javascript:void(0)"> <img src="/images/layer-close.png" width="21" height="21" alt="" /> </a> </div> <h5 class="layer-title">下载全文</h5> <div id="downloadinfo"></div> </div> <div class="layer-box download" id="downloadlayer2" style="display:none;min-height:100px;width:500px;"> <p> <img src="/images/loading2.gif" width="64" height="64"> <span style="font-size:20px;vertical-align:middle;padding-bottom:50px;">正在链接到云南高校图书馆文献保障联盟下载...</span> </p> </div> <script id="bdshare_js" data="type=tools&uid=0" type="text/javascript"></script> <script type="text/javascript"> $(function () { var rd = GetQueryString("rd"); if (rd == "readdown") { var val = $("#hiddowninfo").val(); showdown(val); } $("#bdshare_js").attr("src","http://bdimg.share.baidu.com/static/js/bds_s_v2.js?cdnversion="+Math.ceil(new Date() / 3600000)); }) </script> <div class="layer-bg" style="display: none;"></div> <script type="text/javascript" src="/scripts/login.min.js"></script> <div class="layer-box login" id="divLoginBox" style="display: none;"> <div class="close"><a href="javascript:void(0);" id="btnLoginClose"> <img src="/images/layer-close.png" width="21" height="21" alt="" title="关闭" /></a></div> <h5 class="layer-title">用户登录</h5> <div class="layer-cnt"> <div class="search-list adv-object"> <div class="login-alert" id="divLoginError"></div> <div class="sinput"> <div class="input"><span class="user-ico"></span> <input type="text" id="txtLUsername" name="txtLUsername" maxlength="20"></div> </div> <div class="sinput"> <div class="input"><span class="psw-ico"></span> <input type="password" id="txtLPassword" name="txtLPassword" maxlength="20"></div> </div> <div class="submit"> <input type="button" value="登录" onclick="return normalLogin(loginAfter)" name="" class="btn-s"><input value="IP登录" onclick=" return ipLogin(loginAfter)" type="button" class="btn-c"> </div> </div> </div> </div> <div id="footer"> <p> 云南高校图书馆联盟文献共享服务平台 版权所有© <script type="text/javascript"> document.write(new Date().getFullYear()); </script> </p> <p> 您的IP：216.73.216.231 </p> </div> <input id="hifBaseLog" type="hidden" value="OTExNzFYOzEwNzYzOTU2O1I1NzUuNjswOzsvYXJ0aWNsZS9kZXRhaWwuYXNweA=="/> <script type="text/javascript"> $(document).ready(function () { $("#loginLoading").load("/user/loginheader.aspx?" + Math.random()); }); </script> <script> var _hmt = _hmt || []; (function () { var hm = document.createElement("script"); hm.src = "//hm.baidu.com/hm.js?36a05ff8f5e8a5be3ff03e1d75c8a9d1"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); </script> <script> (function (i, s, o, g, r, a, m) { i['GoogleAnalyticsObject'] = r; i[r] = i[r] || function () { (i[r].q = i[r].q || []).push(arguments) }, i[r].l = 1 * new Date(); a = s.createElement(o), m = s.getElementsByTagName(o)[0]; a.async = 1; a.src = g; m.parentNode.insertBefore(a, m) })(window, document, 'script', '//www.google-analytics.com/analytics.js', 'ga'); ga('create', 'UA-2219013-37', 'auto'); ga('send', 'pageview'); </script> </body> </html>