CD44v6和p53基因分子在肺癌组织中的表达及临床意义  

Expression and Clinical Significance of CD44v6 and p53 Gene in Lung Cancer

作　　者：卓安山[1] 杨秋佳[2] 李奕[1] 

机构地区：[1]中国人民解放军海军411医院呼吸科,上海200081 [2]吉林军医学院附属医院病理科,吉林132013

出　　处：《中国临床医学》2004年第4期653-654,共2页Chinese Journal of Clinical Medicine

摘　　要：目的 :探讨CD4 4v6和p5 3基因分子在肺癌组织中的表达及意义。方法 :采用免疫组织化学染色法 ,对 10 8例经手术切除和 /或肺穿刺活检证实的肺癌组织标本进行CD4 4v6和p5 3基因表达率测定。结果 :① 10 8例肺癌组织中CD4 4v6和p5 3基因表达阳性率分别为 5 6 .6 %和 5 0 .9% ,而正常组织中无一例阳性表达 ;②CD4 4v6基因在非小细胞肺癌组织中的表达率 (6 2 .1% )明显高于小细胞肺癌 (33.3% ) (χ2 =6 .91,P <0 .0 1)。p5 3基因在小细胞肺癌组织中的表达率 (71.4 % )明显高于非小细胞肺癌(4 6 .0 % ) (χ2 =3.90 ,P <0 .0 5 ) ;③有淋巴结转移者CD4 4v6基因表达阳性率 (6 7.6 % )明显高于无淋巴结转移者 (35 .1% ) (χ2 =9.15 ,P <0 .0 1) ,晚期肺癌 (III/IV期 )组织中CD4 4v6基因表达率 (6 5 .7% )高于较早期肺癌 (4 1.5 % ) (χ2 =7.0 9,P <0 .0 1)。而p5 3基因表达与肺癌分期及淋巴结转移无关。结论 :测定肺癌组织中CD4 4v6和p5 3基因表达对肺癌诊断和鉴别诊断具有重要意义。CD4 4v6阳性表达是判断肺癌转移的重要指标之一 ,p5Objective: To explore the expression and clinical significance of CD44v6 and p53 gene in the tissues samples of patients with lung cancer. Methods: CD44v6 and p53 gene expression in the tissues samples obtained by operation and/or lung biopsy in 108 patients with lung cancer was detected by immunohistochemistry SABC method. Results: (1) The positive rate of CD44v6 and p53 gene expression in the samples of lung cancer was respectively 56.5%(61/108) and 50.9%(55/108). There was no expression in the samples of 20 healthy in individuals; (2) The positive rate of CD44v6 expression was markedly higher in NSCLC (62.1%) than that in SCLC (33.3%) (P<0.01), The positive rate of p53 gene expression was significantly higher in SCLC (71.4%) than that in NSCLC (46.0%); (3) The positive rate of CD44v6 expression was more higher in patients with lymph node metastasis (67.6%) than that without lymph node metastasis (35.1%) (P<0.01). The positive rate of CD44v6 expression was higher in stage III+IV patients (65.7%) than those in stage I+II (41.5%).The positive rate of p53 gene was not related to TNM stage and lymph node metastasis of lung cancer. Conclusion:CD44v6 and p53 gene expression may be involved in the progression and metastasis of lung cancer and prognosis of patients with lung cancer.It might be helpful to evaluate the progression of lung cancer, and to predict the metastasis and progression of lung cancer.

关 键 词：CD44V6 P53 肺癌 免疫组织化学 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]