胰岛素对大鼠脑缺血再灌注期细胞凋亡的干预  

INSULIN EFFECT ON APOPTOTIC CELLS DURING REPERFU-SION AFTER ISCHEMIA IN RAT

作　　者：苏庆杰[1] 黄海威[2] 肖小华[2] 黄如训[2] 

机构地区：[1]海南医学院附属医院神经内科,海口570102 [2]中山大学第一医院神经科,广州510080

出　　处：《海南医学院学报》2004年第4期214-216,共3页Journal of Hainan Medical University

基　　金：美国中华医学基金会(CMB)资助(NO:93~594)

摘　　要：目的:研究胰岛素对减轻脑缺血再灌注损伤的作用机制。方法:制备肾血管性高血压大鼠(RHR),用线栓法复制大脑中动脉阻塞(MCAO),造成缺血2h后分别再罐注1,3,5d,并于再罐注后开始使用胰岛素,采用TUNEL法原位标记DNA片段,检测凋亡细胞的变化。结果:实验组TUNEL阳性细胞明显减少(P<0.05)。结论:胰岛素减轻脑缺血再灌注损伤的机制与减少再灌注后细胞凋亡或死亡有关。Objective: To study the mechanism of insulin treatment on reperfusion injury after ischemia. Methods: The renovascular hypertension rat(RHR) were don ed to MCAO as described by Zea Longa for 2 hours and treated immediately with insulin after reperfulsion. The a poptotic cells were detected at 1,3,5 days after reperfusion with TUNEL method. Results: The numbers of the T UNEL positive cells were significantly lower in the tested groups than the controlled groups(P < 0 .05). Conclusion: Inhibitting apoptosis after reperfusion is probably one of the mechanisms that insulin reduc es reperfusion injury.

关 键 词：胰岛素 大鼠 脑缺血 再灌注损伤 细胞凋亡 作用机制 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R977.15[医药卫生—临床医学]