17β-雌二醇对人成骨样细胞OS-732增殖和分化的影响  

Effect of 17β-estradiol on the proliferation and differentiation of human osteoblast-like cells OS-732

作　　者：高秀辉[1] 薛延[1] 袁润英[1] 相东[1] 王红霞[1] 郑铭豪[1] 颜卉君[1] 

机构地区：[1]北京积水潭医院创伤骨科研究所生化室,北京积水潭医院创伤骨科研究所免疫室,西澳大学外科系,北京师范大学生物系

出　　处：《中国骨质疏松杂志》1997年第4期6-10,共5页Chinese Journal of Osteoporosis

摘　　要：采用绘制生长曲线３Ｈ－ＴＤＲ掺入、ＡＬＰ、ＢＧＰ的定量测定、免疫组化、原位杂交等和光镜等方法，探讨了１７β－雌二醇对ＯＳ－７３２细胞增殖和分化的影响及对骨粘连蛋白（ＯＮ）ｍＲＮＡ表达的影响。结果显示：１７β－雌二醇以剂量依赖性促进ＯＳ－７３２细胞的增殖，其中以１０－８ｍｏｌ／Ｌ浓度作用最强；１７β－雌二醇可以轻度降低ＡＬＰ的活性和ＢＧＰ的分泌，对ＯＳ－７３２细胞分化有轻微抑制作用。免疫组化实验结果表明ＯＳ－７３２细胞上存在ＥＲ受体，且给药后核内ＥＲ增多，为雌激素直接作用于成骨样细胞提供了一个例证。原位杂交结果表明ＯＳ－７３２上存在（ＯＮ）ｍＲＮＡ的表达，而给药后无明显变化。The aim of this study was to understand the effect of 17-β-estradiol (E2)on the proliferation and differentiation of human osteoblast-like cells OS-732. We found that 17β-estradiol treatment on OS-732 cells stimulated cell proliferation and 3H thymidine incorporation in dose dependent manner. The maximal effect was seen in 10 8 mol/L of 17β-estradiol. 10-8 mol/L of 17β-estradiol treatment on day 4caused a slightl decrease of cellular alkaline phosphatase (ALP)activity and the levels of osteocalcin (BGP) in cell culture measured by chemical analysis,ELISA and cell staining. The results showed that estrogen on receptors (ER) could be detected in OS-732 cells by immunochernical staining and they increased after E2 treatment. And there was no effect of estrogen expression of osteonectin (ON) mRNA by in situ hybridization using biotin-labelled dUTP. our study suggests that estrogen stimulates the proliferation and slightly inhibits the differentiation of OS-732 cells.

关 键 词：成骨样细胞 17Β-雌二醇 ER 原位杂交 给药 免疫组化 分化 增殖 ^3H-TDR掺入 影响 

分 类 号：R737[医药卫生—肿瘤] R735[医药卫生—临床医学]