六位点微卫星组合用于膀胱癌诊断的研究  

Study of the panel of 6 microsatellites in diagnosis of bladder cancer

作　　者：赵军[1] 贺大林[1] 杨林[1] 何辉[1] 南勋义[1] 

机构地区：[1]西安交通大学泌尿外科研究所西安交通大学第一医院泌尿外科,710061

出　　处：《中华泌尿外科杂志》2004年第9期616-618,共3页Chinese Journal of Urology

基　　金：卫生部自然科学基金 ( 98 2 0 5 );卫生部临床学科重点项目 ( 2 0 0 12 0 2 9)

摘　　要：目的　探讨 6个微卫星位点组合在膀胱肿瘤诊断中的意义。　方法　选择 10个微卫星位点 (D9S16 2、D9S171、IFNA、D9S176、D9S195、D14S5 1、ACTBP2、D14S2 6 7、D17S6 95、D2 1S12 4 5 ) ,应用PCR SSLP法检测 32例膀胱肿瘤患者的肿瘤组织、膀胱冲洗液沉渣微卫星改变 ,15例非膀胱肿瘤患者为对照组。　结果　 32例膀胱肿瘤组织中 ,微卫星改变阳性 30例 ,敏感性为 93.8% (30 / 32 ) ,对照组膀胱冲洗液沉渣微卫星改变均为阴性。微卫星改变与膀胱肿瘤分期、分级无相关性。选取微卫星改变发生率最高的D9S171、IFNA、D14S5 1、D17S6 95、D2 1S12 4 5、ACTBP2 6位点组合微卫星改变阳性率为 90 .6 % (2 9/ 32例 ) ,与 10个微卫星位点组合相比差异无显著性意义。　结论　此 6个微卫星位点组合检测膀胱肿瘤可能具有较高的敏感性和特异性。Objective To evaluate the significance of the panel of 6 microsatellites in detection of bladder cancer. Methods In the tumor tissue and urine sediment of 32 cases of bladder cancer 10 microsatellites were chosen and PCR-SSLP silver staining assay was conducted according to the methods described in the literature and our previous study.15 cases of non-bladder cancer served as controls. Results Microsatellite alternate (MA) was found in 30 out of 32 cases of bladder cancers,the sensitivity being 93.8%.The MA of urine sediment of 15 cases of non-bladder cancer was negative,the specifity being 100.0%.Among the 10 microsatellites, 6 ones were chosen;the MA positivity of the panel of the 6 ones was 90.6% (29/32).This result was not significantly different from that of the panel of 10 microsatellites. Conclusions MA assay is a sensitive,effective method for detection of bladder cancer.Compared with the panel of 10 microsatellites,the panel of 6 microsatellites may be a better tool for detection of bladder cancer.

关 键 词：六位点微卫星 膀胱癌 肿瘤 临床资料 

分 类 号：R737.14[医药卫生—肿瘤]