DABS-Cl 试剂在反相高效液相色谱中的应用  

Application of DABS-C(?) reagent In reverse-phase high performance liquid chromatography

作　　者：费达[1] 江国荣 李勇健 任光荣 

机构地区：[1]苏州大学分析测试中心,苏州215006 [2]苏州中医研究所,苏州215003

出　　处：《化学试剂》1993年第1期42-44,共3页Chemical Reagents

摘　　要：选定了两套完全不同的色谱条件,并对 DABS-氨基酸进行了高效液相色谱分析实验。DABS-Cl 与氨基酸的衍生反应所需的时间不足 lh。The DABS-Cl(4-dimethylaminoazobenzene-4'-sulf-onyl chloride)is a sort of chromophoric labelling reagent em-ployed for labelling of amino acids,polypeptides,and pro-teins.With the DABS-Cl precolumn derivatization techni-que,the above mentioned compounds are easily separated byhigh performance liquid chromatography,and then deter-mined spectrophotometrically at 436nm at the picomole(10^(-12)mol)level.The DABS derivatives are highly stable,the derivatization procedure is relatively simple and fairlyrapid,the whole procedure takes less than one hour.

关 键 词：DABS-C1 氨基酸 反相 高效液相色谱 

分 类 号：O657.72[理学—分析化学]