蛋白激酶A RIα亚单位与肺癌临床病理特征的相关性研究  

Relationship between overexpression of the RIα subunit of the cAMP-dependent protein kinase and clinicopathological features of lung cancer

作　　者：石素胜[1] 何祖根[1] 邵康[2] 周芳[2] 熊美华[2] 黄微[2] 姆巴东[2] 张翠艳[2] 张森[2] 孙耘田[1] 赫捷[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院病理科,北京100021 [2]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院胸外科,北京100021

出　　处：《中华肿瘤杂志》2004年第9期547-550,共4页Chinese Journal of Oncology

基　　金：国家科技攻关计划基金资助项目 ( 2 0 0 2BA711A0 6)

摘　　要：目的　探讨蛋白激酶A(PKA)RIαmRNA表达与肺癌临床病理特征的相关性。方法　采用逆转录 聚合酶链反应 (RT PCR)的方法 ,检测 5 4例肺癌患者癌组织及相应远端正常组织中PKARIαmRNA的表达水平。结果　 (1)PKARIαmRNA在肺癌组织中的表达 (6 6 .7% )明显高于相应远端正常组织 (2 0 .4 % ,P <0 .0 1)。 (2 )PKARIαmRNA表达与肺癌TNM分期明显相关 (P <0 .0 1) ,临床分期级别越高 ,PKARIαmRNA表达越高。 (3)淋巴结转移阳性者的PKARIαmRNA表达水平 ,明显高于淋巴结转移阴性者 (P <0 .0 1)。 (4 )PKARIαmRNA表达与肿瘤组织学类型、分化及肿瘤大小无相关性(P >0 .0 5 )。结论　PKARIαmRNA在肺癌组织中过表达 ,提示PKARIαmRNA在肺癌的发生、发展。Objective To explore the relationship between the overexpression of PKA RIα mRNA and cliniopathological parameters in lung cancer. Methods RT-PCR was used to detect the expression of PKA RIα mRNA in 54 cases with human lung cancer and matched normal tissues. Results (1) The expression of PKA RIα mRNA was significantly higher in cancer tissue (66.7%) than in normal tissues (20.4%) (P<0.01). (2) The expression was significantly correlated with TNM stage (P<0.01), being increased with TNM stage. (3) The expression was significantly higher in patients with positive lymph nodes than in those with negative lymph nodes (P<0.01). (4) There were no significant associations of PKA RIα mRNA expression with histological type, differentiation grade or size of the tumor. Conclusion This study indicates that the overexpression of PKA RIα mRNA may play an important role in the progression, metastasis and prognosis of lung cancer.

关 键 词：RI mRNA表达 肺癌 PKA 临床病理特征 蛋白激酶A 正常组织 表达水平 分化 亚单位 

分 类 号：R734.2[医药卫生—肿瘤] R735[医药卫生—临床医学]