两种生长因子对牙周膜细胞表达骨保护因子的影响  

Insulin-like Growth Factor-Ⅱ and Basic Fibroblast Growth Factor Affect Periodontal Ligament Cells Expressing Osteoprotegerin in vitro

作　　者：凌均棨[1] 李京平[1] 

机构地区：[1]中山大学光华口腔医学院,广东广州510060

出　　处：《华西口腔医学杂志》2004年第5期366-369,共4页West China Journal of Stomatology

摘　　要：目的 观察胰岛素样生长因子-Ⅱ(IGF-Ⅱ)和碱性成纤维细胞生长因子(bFGF)对牙周膜细胞增殖及分泌骨保护因子(OPG)的影响。方法 培养牙周膜细胞并形成单细胞克隆,培养基中加入bFGF和IGF-Ⅱ,MTT法检测牙周膜细胞增殖水平,Sandwich ELISA测定培养上清液中OPG含量。结果IGF-Ⅱ促进牙周膜细胞增殖但降低细胞OPG的表达量(P<0.05)。bFGF也促进牙周膜细胞增殖(P<0.05),但对细胞表达OPG的影响作用不明显(P>0.05)。结论 应用细胞因子促进牙周再生时应考虑其对牙周骨组织平衡的影响。Objective This study was carried out to investigate the effects of insulin-like growth factor-Ⅱ(IGF- Ⅱ ) and basic fibroblast growth factor (bFGF) on osteoprotegerin(OPG) secretion of periodontal ligament cells (PDLCs) .Methods Healthy human premolars extracted for orthodontic reasons from 12-14 years old donators were obtained, and periodontal tissues were collected and cultured to obtain PDL cells. Primary or first passage PDLCs were cloned by means of limited dilutions. PDLCs with os-teoblastic phenotypes were characterized as follows: Alkaline phosphatase activity, collagen Ⅲ production and bone-like nodules formation. IGF- Ⅱ and bFGF were added into culture media and their effects on PDLCs proliferation and OPG secretion were observed. The OPG concentrations in cell culture supernatants were detected by sandwich ELJSA. Living cell numbers were demonstrated by MTT test. The average levels of OPG secretion by a single cell were calculated by dividing OPG concentration with MTT-test result. Results Both IGF- Ⅱ and bFGF upregulated the mtt values ( P < 0.05 ), but IGF- II downregulated the opg/mtt values ( P < 0.05), whereas bFGF had no significant effect on opg/mtt values ( P > 0.05). Conclusion IGF- II enhances the proliferation of PDL cells but prohibits OPG secretion. Although bFGF has the same effect on the proliferation of PDL cells, it has no effect on OPG secretion. Before cytokines were used to enhance periodontal regeneration, their effects on local bone balance should also be studied.

关 键 词：牙周膜 细胞培养 碱性成纤维细胞生长因子 胰岛素样生长因子 

分 类 号：R781.4[医药卫生—口腔医学]