土槿乙酸诱导HL60细胞凋亡及其与半胱氨酸蛋白酶-3活化的关系  

Apoptosis and Activation of Caspase-3 in Leukemia HL60 Cells Induced by Pseudolaric Acid B

作　　者：徐瑞成[1] 刘燕青[1] 买霞[1] 范磊[1] 徐丽君[1] 陈虹[2] 

机构地区：[1]武警医学院细胞生物学教研室,300162 [2]武警医学院药学教研室,300162

出　　处：《实用癌症杂志》2004年第4期344-346,350,共4页The Practical Journal of Cancer

基　　金：国家自然科学基金资助项目 (30 1 71 1 0 8)

摘　　要：目的　探讨土槿乙酸 (PLAB)的抗癌活性及其机制。方法　对用不同浓度PLAB处理的HL 60细胞 ,或半胱氨酸蛋白酶 3 (caspase 3 )抑制剂DEVD fmk预处理的HL 60细胞。采用MTT法检测HL60细胞抑制增殖作用 ,采用Hoechst3 3 3 42 /PI双荧光活细胞染色和原位末端标记 (TUNEL)方法检测细胞凋亡 ,CPP3 2colorimetrickit检测caspase 3酶活性。 结果　PLAB以剂量依赖的方式抑制HL60细胞的生长 ,其IC50 值为 1μmol/L。PLAB诱导HL60细胞死亡的形式以细胞凋亡为主 ,且该凋亡可被caspase 3抑制剂所遏制。PLAB诱导的caspase 3酶的活化与剂量和作用时间呈依赖关系。 结论　PLAB能有效诱导HL 60细胞凋亡 ,该凋亡过程伴有caspaseObjective To study the anti-tumor effect of pseudolaric acid B(PLAB) and the mechanism.Methods Leukemia HL60 cells were treated with PLAB at different concentrations.Anti-proliferative effect of PLAB was studied by MTT assay.Cell apoptosis was assessed by Hoechst33342/PI dye staining and TdT-mediated dUTP nick-end labeling(TUNEL) staining.Caspase-3 activity was determined by CPP32 colorimetric assay kit.Results PLAB inhibited the growth of HL60 cells in a dose-dependent manner,with the IC_(50) of 1 μmol/L.PLAB caused extensive nuclear condensation,DNA fragmentation and cell death.Pretreatment with caspase-3 specific inhibitor DEVD-fmk prevented PLAB-induced apoptosis.PLAB activated caspase-3 in HL60 cells in a dose-and time-dependent manner.Conclusion PLAB can efficiently induce apoptosis in HL60 cells and the activation of caspase-3 may contribute to the effect.

关 键 词：HL60细胞 细胞凋亡 诱导 CASPASE-3抑制剂 半胱氨酸蛋白酶-3 剂量 CPP32 活细胞 荧光 增殖作用 

分 类 号：R733[医药卫生—肿瘤] R735[医药卫生—临床医学]