化学毁损伏隔核对大鼠学习记忆能力与探索行为的影响  

Effects of nucleus accumbens septi by chemical injury on learning memory abilities and exploratory behavior in rats

作　　者：邵云[1] 王旭梅[1] 魏孝琴[2] 薛一雪[2] 李文彦[2] 蔡葵[2] 

机构地区：[1]中国医科大学附属第二医院心理卫生科,辽宁省沈阳市110004 [2]中国医科大学神经生物学教研室,辽宁省沈阳市110001

出　　处：《中国临床康复》2004年第31期6882-6884,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察双侧毁损伏隔核对大鼠学习记忆能力和探索行为的影响,为现代主体定向技术毁损脑内核团治疗难治性精神疾病提供参考。方法:在脑立体仪的引导下,用微量加样器将6-羟多巴胺缓慢注射入所有毁损组大鼠双侧伏隔核,而所有对照组注射等量生理盐水。以Morris水迷宫实验为评价指标(毁损组与对照组各10只,毁损前后自身对照10只),评价双侧毁损伏隔核对大鼠空间学习记忆能力的影响;以场地探索实验为评价指标(毁损组与对照组各11只),评价双侧毁损伏隔核对大鼠探索行为的影响。结果:脑切片染色显示,6-羟多巴胺能够毁损伏隔核中的多巴胺系统。在水迷宫实验中,无论是毁损组与对照组相比,还是毁损前后自身对照差异均无显著性(P>0.05)。场地探索实验中毁损组A室直立数,直立时间前2d低于对照组,其运动方格数却高于对照组,差异均有著性(P<0.05～0.01);而毁损组大鼠进入B室潜伏期却高于对照组,并且毁损组大鼠对新物的探索时间高于对照组,差异均有著性(P<0.05～0.01)。第9天撤除新物后毁损组大鼠对新物的探索时间却低于对照组(P<0.05)。结论:化学毁损伏隔核中的多巴胺系统能够影响对大鼠的空间记忆形成,再获得及空间定位能力无明显影响,但对大鼠运动探索能力有一定影响,毁损组大鼠在行为灵活性和转换性上存在缺陷。AIM:To investigate the effects of nucleus accumbens septi(NAS) by bilateral injury on learning memory abilities and exploratory behavior in rats,and offer references to treat failed mental disease with nuclear mass injury in brain induced by modern stereotactic technology. METHODS:Under the guidance of the brain solid positioner,6-hydroxydopamine was slowly injected into the bilateral NAS of all rats in injured group with a micropipette,but rats in control group were injected with equivalent saline.The effects of bilateral injured NAS on spatial learning memory were evaluated by means of Morris water maze test with ten rats in injured group,control group and auto-control group before and after injury respectively.The effects of bilateral injured NAS on exploratory behavior were evaluated by means of compartment investigation test with eleven rats in injured group and control group respectively. RESULTS:Staining slices of brain showed that the dopamine systems in NAS could be destroyed by 6-hydroxydopamine.There was no significant difference whether between the injured group and the control group or the auto-control before and after injury in the water maze test(P >0.05).Compartment investigation experiment showed that upright amount and upright time in A compartment two days ago were lower in injured group than those in control group,but the motor-quadrat amount was higher in injured group than that in control group,which was significant difference(P< 0.05 to 0.01).The latency in B compartment and the explorative time for new things of rats were higher in injured group than those in control group,which was significant difference(P< 0.05 to 0.01).The explorative time for new things of rats was lower in injured group than that in control group after the movement of new things on the ninth day(P< 0.05). CONCLUSION:Chemically injured dopamine systems in the NAS can affect the formation and the recapacitation of spatial memory,and it has no effects on the spatial localizing ability.However, it can affect explorativ

关 键 词：毁损 大鼠 对照组 伏隔核 双侧 学习记忆能力 行为 化学 探索实验 探索能力 

分 类 号：R749[医药卫生—神经病学与精神病学] R338[医药卫生—临床医学]