伊达拉奉对沙土鼠全脑缺血再灌注损伤后海马CAI区神经元凋亡相关基因Bcl-2、Bax表达的影响  

Study on Effect of Edaravone on Expression of Apoptosis-related gene: Bcl-2, Bax in Hippocampal CAI Region after Cerebral Ischemia/Reperfusion in Rats

作　　者：高阳[1] 吴婷[1] 丁新生[1] 印卫兵[1] 龚洁[1] 李娟[1] 高飞[1] 

机构地区：[1]南京医科大学第一附属医院神经内科,南京210009

出　　处：《江苏大学学报（医学版）》2004年第5期384-385,388,F006,共4页Journal of Jiangsu University：Medicine Edition

摘　　要：目的 :探讨伊达拉奉对沙土鼠全脑缺血再灌注损伤后海马CAI区神经元凋亡相关基因Bcl 2、Bax表达的影响及可能的作用机制。方法 :采用Global模型造成沙土鼠全脑缺血损伤模型 ;腹腔注射伊达拉奉对其进行治疗后运用免疫组织化学方法 ,检测受损脑组织海马CAI区神经元中的Bcl 2、Bax免疫反映阳性细胞数量。结果 :治疗组大鼠脑组织中Bcl 2表达明显增加 ,Bax表达明显低于损伤对照组 (P <0 0 5 )。结论 :伊达拉奉通过清除羟自由基 ,抗脂质过氧化 ;可能通过增强Bcl 2的表达 ,抑制Bax的表达来减少脑缺血后神经细胞的凋亡 ,发挥脑保护作用。s] Objective: To discuss the effect and possible me ch anism of Edaravone on expression of apoptosis-related gene: Bcl-2, Bax in hippocampal CAI region after cerebral ischemia in gerbils. Methods:Gerbils were treated with Edaravone injection into abdominal cavity after cere bral ischemia/reperfusion(I/R) and then immunohistochemistry was used to detec t the expression of Bcl-2,Bax in neuron of hippocampal CAI region. R esults: Compared with the control group,the expression of Bcl-2 in hippocampal CAI region increased obviously while the expression of Bax decreased in treated group. Conclusion:Edaravone may red uce the apoptosis of neuron after I/R through enhancing the expression of Bcl -2 and inhibiting the expression of Bax.But its detailed mechanism is sti ll in need for further research. [

关 键 词：全脑缺血 伊达拉奉 凋亡 BCL-2基因 BAX基因 

分 类 号：R-33[医药卫生]