釉基质蛋白对体外培养的兔骨髓基质细胞生物学功能的影响  

Effects of Enamel Matrix Proteins on Some Special Properties of Bone Marrow Stromal Cells in vitro

作　　者：杜岩[1] 吴织芬[1] 董广英[1] 万玲[1] 侯锐[1] 

机构地区：[1]第四军医大学口腔医学院牙周黏膜病科,陕西西安710032

出　　处：《口腔医学研究》2004年第5期457-461,共5页Journal of Oral Science Research

摘　　要：目的 :观察釉基质蛋白对兔骨髓基质细胞增殖、碱性磷酸酶活性、总蛋白合成等一般生物学功能的影响。方法 :MTT法检测细胞增殖能力 ,酶动力法检测细胞碱性磷酸酶活性 ,利用Biophotometer生物检测仪检测细胞总蛋白合成能力。结果 :釉基质蛋白可以促进骨髓基质细胞的增殖、提高细胞的碱性磷酸酶活性和总蛋白合成能力 ,以 10 0mg/L釉基质蛋白的促进作用最明显。釉基质蛋白促进细胞增殖、提高细胞碱性磷酸酶活性、促进细胞总蛋白合成的作用分别在 12 0h、72h、16 8h时最明显。结论 :釉基质蛋白对兔骨髓基质细胞增殖、碱性磷酸酶活性。Objective: In this experiment, some special properties of bone marrow stromal cells (BMSCs) are investigated such as proliferation, alkaline phosphate (ALP) activity, and total protein synthesis ability. Methods: BMSCs' proliferation, ALP activity and total protein synthesis ability was measured separately by MTT method, enzyme dynamic method, Biophotometer. Results: EMPs at certain concentrations: enhanced proliferation of BMSCs; improved ALP activity in BMSCs; increased total protein production by BMSCs. 100 mg/L is the best concentration that had the strongest promotion of BMSCs' special properties. The promotion ability was significant at 120 h, 72 h, 168 h for proliferation, ALP activity and total protein synthesis of BMSCs separately. Conclusion: EMPs can act as a kind of promotive factor for BMSCs.

关 键 词：釉基质蛋白 骨髓基质细胞 增殖 碱性磷酸酶 总蛋白 

分 类 号：R781.4[医药卫生—口腔医学]