超声波对古尼虫草菌丝体多糖免疫调节活性的影响  

The Effect of Ultrasonication on Immuno-modulating Activity of Mycelial Polysaccharide from Cordyceps gunnii

作　　者：肖建辉[1] 梁宗琦[2] 刘爱英[2] 万卫红[1] 肖瑜[1] 刘金伟[1] 

机构地区：[1]遵义医学院附属医院贵州省细胞工程重点实验室,遵义563003 [2]贵州大学真菌资源研究室,贵阳550025

出　　处：《中药材》2004年第10期747-750,共4页Journal of Chinese Medicinal Materials

基　　金：贵州省赤天化集团公司重点资助虫草开发项目(2 0 0 1 10 19)

摘　　要：目的 :研究古尼虫草菌丝体多糖经超声波解聚前后的免疫调节活性。方法 :用超声波解聚古尼虫草菌丝体多糖 ,并分别采用MTT法、中性红比色法测定古尼虫草菌丝体多糖及其解聚物对小鼠脾淋巴细胞增殖、腹腔巨噬细胞 (PMΦ)吞噬功能以及细胞毒T淋巴细胞 (CTL)杀伤活性的影响。结果 :解聚后的古尼虫草菌丝体多糖对小鼠脾淋巴细胞增殖、PMΦ吞噬功能以及CTL活性的免疫抑制效果明显增强 ,尤其在 10 μg/ml和 10 0 μg/ml剂量时的抑制效果更好。古尼虫草菌丝体多糖并不随超声波解聚时间的延长而提高其免疫抑制活性 ,解聚 1～ 2h和 4～ 8h的抑制效果没有显著的差异。结论 :古尼虫草菌丝体多糖经超声波解聚后有更强的免疫调节活性 ,其解聚时间以 1～ 2h为宜。Objective: To investigate immuno-modulating activity change after mycelial polysaccharide from Cordyceps gunnii(MPCG) was depolymerized by ultrasonication. Methods: The effects of MPCG and its depolymerizing mixture on mice spleen lymphocytes proliferation, mice peritoneal macrophage(PMΦ) phagocytosis and cytotoxin T lymphocytes (CTL) activity were studied by MTT method, neutral red colorimetry, respectively. Results: In vitro, MPCG depolymerized by ultrasonication could more significantly inhibite spleen lymphocytes proliferation, PMΦ phagocytosis of neutral red and CTL activity in mice than MPCG, in particular at doses of 10 μg/ml and 100 μg/ml. Whereas, four kinds of depolymerized mixture depolymerized for 1h to 2h and 4h to 8h by ultrasonication, respectively, had not evident differences at three various doses. Conclusion: MPCG was suitable for depolymerizing for 1h to 2h and its depolymerized mixture had stronger immuno-modulating activity than MPCG.

关 键 词：古尼虫草 免疫调节活性 菌丝体多糖 脾淋巴细胞增殖 吞噬功能 小鼠 超声波 解聚 增强 研究 

分 类 号：R284[医药卫生—中药学] R282.71[医药卫生—中医学]