绿康银杏叶片质量标准初步研究  

Studies on the Quality Standard of "Lü Kang Yin Xing Ye Pian"

作　　者：陶玲[1] 黄芳 吴惠勤[2] 施文平[1] 师晓嘉[1] 

机构地区：[1]中山大学附属第三医院,广州510630 [2]广州分析测试中心,广州510070

出　　处：《中药材》2004年第10期775-778,共4页Journal of Chinese Medicinal Materials

基　　金：广东省重点科技攻关〔(1997) 114〕资助项目

摘　　要：目的 :研究并制定绿康银杏叶片的质量控制方法。方法 :采用薄层色谱法对绿康银杏叶片中的有效成分银杏叶内酯进行定性鉴别 ,采用GC/MS法测定其中紫苏子脂肪油中的脂肪酸组成 ,并用高效液相色谱法测定银杏黄酮、银杏内酯的含量 ,对其中限量的银杏酸进行了测定。结果 :精密度试验RSD为 1 2 %～ 2 5 %(n =5 ) ,稳定性试验 2 4h内稳定不变 ,峰面积与其浓度线性关系良好 (r值均为 0 999以上 ) ,平均回收率均为 95 %以上。结论 :本法结果准确 ,可作为该制剂的质量控制方法。Objective: The quality control method of 'Lü Kang Yin Xing Ye Pian' (LKYXYP) was studied and established. Method: The ginkgolides in LKYXYP were identified by TLC. The compounds of fatty acids in Perillae oil were determined by GC/MS. Flavones, ginkgolides and ginkgolic acids were determined by HPLC. Result: There were good linears relationship between the peak area and concentration, r=0.999, RSD=1.2%～2.5%(n=5), and recoveries of each composition was above 95%, the contation was stable in 24h.Conclusion: This method is proved to be accurate and able to be applied for the quality control of this preparation.

关 键 词：银杏叶片 初步研究 精密度试验 质量控制方法 银杏内酯 定性鉴别 银杏酸 平均回收率 脂肪酸组成 浓度 

分 类 号：R286.0[医药卫生—中药学] R284[医药卫生—中医学]