酒精对小鼠神经母细胞发育的影响  

Effect of alcohol on the development of mouse neuroblasts

作　　者：许雅君[1] 柳鹏[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系

出　　处：《中国生育健康杂志》2004年第4期221-225,共5页Chinese Journal of Reproductive Health

基　　金：国家自然科学基金资助项目 (No. 3 0 2 713 64 )

摘　　要：目的　探讨酒精对胚胎神经母细胞发育的影响及其与畸形发生之间的联系。　方法　从 12 d龄、体节数 34～ 36的小鼠胚胎分离中脑神经母细胞接种培养板 ,给予不同剂量的酒精于体外连续培养 5 d后 ,检测酒精对细胞增殖和分化的影响 ,及其线粒体膜电位的变化和细胞凋亡情况。　结果　酒精对中脑神经母细胞增殖和分化均有抑制作用 ,其分化抑制剂量 ( ID50 )和增殖抑制剂量( IP50 )分别为 2 10 .2 9m M和 336 .5 5 m M,分化比增殖更敏感。随酒精染毒剂量的增加 ,细胞线粒体膜电位呈下降趋势 ,凋亡和坏死细胞数量逐渐增加 ,存在剂量 -反应关系。Objective To investigate the effect of alcohol on the development of embryonic neuroblasts and the relation to alcohol's teratogenicity. Methods Micromass cell culture was used to explore the effect of alcohol on the development of mouse midbrain neuroblasts.Cells were cultured in different alcohol concentrations.Five days later,proliferation,differentiation,membrane potential of mitochondria and cellular apoptosis and death were detected. Results Both proliferation and differentiation of the midbrain neuroblasts were inhibited by alcohol. ID 50 and IP 50 were 210.29 mM and 336.55 mM,respectively.Differentiation was more sensitive than proliferation.As the alcohol concentration increasing,the mitochondrial membrane potential decreased and the number of cells undergoing apoptosis and death increased. Conclusion Alcohol can inhibit the proliferation and differentiation of midbrain neuroblasts.and can induce excessive apoptosis,which may be related to the teratogenic action of alcohol on central nervous system.

关 键 词：酒精 神经 分化 增殖 中脑 线粒体膜电位 凋亡 结论 情况 联系 

分 类 号：R361[医药卫生—病理学]