NF-κB/IκB信号通路在IL-1β诱导的肾系膜细胞环氧化酶-2表达中的作用  

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

作　　者：丁桂霞[1] 张爱华[2] 黄松明[2] 吴元俊[2] 费莉[2] 郭梅[2] 陈荣华[2] 

机构地区：[1]南京医科大学第二附属医院儿科,江苏南京210011 [2]南京医科大学小儿肾脏病研究中心,江苏南京210029

出　　处：《中国病理生理杂志》2004年第10期1754-1758,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .30 10 0 0 81) ;江苏省教委自然科学基金资助项目 (No.99KJB32 0 0 0 3)

摘　　要：目的 :探讨核因子 -κB (NF -κB) /IκB信号通路在肾小球系膜细胞环氧化酶 - 2 (COX - 2 )表达中的作用。方法 :放射免疫测定法检测系膜细胞培养上清中PGE2 水平 ;RT -PCR和Westernblot检测COX - 2表达 ;凝胶电泳迁移率 (EMSA)和Westernblot检测肾小球系膜细胞中NF -κB活化、p6 5亚基核转位以及IκBα和IκBβ的降解。结果 :IL - 1β显著上调系膜细胞PGE2 释放和COX - 2表达 ,PDTC显著抑制IL - 1β诱导的COX - 2表达及PGE2 释放 ;IL - 1β诱导系膜细胞NF -κB活化 ,p6 5核转位及胞浆内IκBα和IκBβ的降解。结论 :IL - 1β通过NF -κB/IκB信号转导通路诱导肾小球系膜细胞中COX -AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of (cyclooxygenase-2) (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE_2 concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE_2 production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway. [

关 键 词：肾 系膜细胞 NF-KAPPA 前列腺素内过氧化物合酶 

分 类 号：R363[医药卫生—病理学]