EGb761增强c-jun表达减少轴突撕脱后运动神经元的死亡  

Ginkgo biloba extract enhances c-jun expression and attenuates motoneuron death induced by root avulsion

作　　者：李方澜[1] 周丽华[1] 袁群芳[1] 姚志彬[1] 吴武田[2] 

机构地区：[1]中山大学中山医学院解剖教研室,广东广州510080 [2]香港大学医学院解剖系

出　　处：《中国病理生理杂志》2004年第10期1783-1785,共3页Chinese Journal of Pathophysiology

基　　金：广东省科委社会发展项目 (No.2 0 0 2C30 111)

摘　　要：目的 :观察EGb76 1对脊神经根撕脱后前角运动神经元c -jun表达和存活的影响。方法 :成年Sprague-Dawley雌性大鼠 180只 ,行脊髓C5 -T1节段神经根撕脱术后 ,每日给予腹腔注射 1mLEGb76 1(2 5mg·kg-1) ,对照组注射同容积的生理盐水。治疗后 4h、12h、1d、3d、5d、1周、2周、4周和 6周 9个时点分别处死动物 ,取C7脊髓节段行c-jun免疫细胞化学和中性红染色。定量比较两组c -jun阳性和存活的运动神经元数目。结果 :对照组损伤侧运动神经元 4h开始表达c-jun ,1d达高峰 ,以后逐渐下降至术后 2周。术后 2周运动神经元开始死亡 ,4周 - 6周达高峰。EGb76 1组各时点c -jun阳性和存活的运动神经元数目都多于对照组。结论 :EGb76 1能有效地减少神经根撕脱后运动神经元的死亡 。AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg·kg^(-1)·d^(-1) or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of (c-jun) positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons. [

关 键 词：二裂银杏 基因 C-JUN 运动神经元 臂丛 撕脱 

分 类 号：R363[医药卫生—病理学]