TGF-β1显著上调其信号蛋白Smad2在腹膜间皮细胞中的表达  

TGF-β1 upregulates Smad2 expression in peritoneal mesothelial cells

作　　者：杨琼琼[1] 余学清[1] 段文娟[1] 李晓燕[1] 黄锋先[1] 

机构地区：[1]中山大学附属第一医院肾内科教育部肾脏病重点实验室,广东广州510080

出　　处：《中国病理生理杂志》2004年第10期1790-1793,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .30 2 70 6 30 ) ;广东省自然科学基金资助项目 (No .2 0 0 0 1380 ) ;广东省医学科研基金资助项目 (No .A2 0 0 115 6 )

摘　　要：目的 :探讨Smad2信号蛋白在腹膜间皮细胞中的表达及转化生长因子 - β1(TGF - β1)对其表达的影响。方法 :大鼠腹膜间皮细胞分别培养于不同浓度TGF - β1培养液 (0、1 2 5、2 5、10 μg/L)中 ,用RT -PCR和免疫组化的方法检测不同时间 (0、5、15、30、6 0、12 0min)Smad2表达的水平和Smad2细胞内定位变化。结果 :Smad2mRNA的表达在TGF - β1刺激后 5min开始升高 ,呈时间依赖性 ,在 30min达到高峰 ,而后逐渐减弱 ,在 12 0min降至接近正常水平 ;Smad2mRNA表达也呈浓度依赖性 ,随TGF - β1浓度的增高而表达增强。磷酸化Smad2蛋白的表达和核内聚集也与mRNA表达一致 ,呈时间和浓度依赖性。Smad2在TGF - β1刺激下从胞浆转位至核内聚集 ,并在 30min达高峰。结论 :腹膜间皮细胞内存在Smad2的表达。TGF - β1可刺激Smad2表达上调和活化 ,转位至核内发挥作用 。AIM: To investigate the expression of Smad2 signal protein in peritoneal mesothelial cells and how transforming growth factor β1 (TGF-β1) affects its expression. METHODS: Rat peritoneal mesothelial cells were cultured in different levels of TGF-β1 (0,1.25,2.5,10 μg/L) for different time (0,5,15,30,60,120 min). Endogenous Smad2 expression was evaluated by RT-PCR and immunohistochemical assay. The alteration of subcellular location of Smad2 was determined by immunohistochemical assay. RESULTS: TGF-β1 induced Smad2 mRNA expression, which increased at 5 min, peaked at 30 min, and declined to baseline levels by 120 min, in a time-dependent manner. Smad2 mRNA expression induced by TGF-β1 was also in a dose -dependent manner. TGF-β1 induced Smad2 phosphorlylation and nuclear localization in both time-dependent and dose-dependent manner, which was concordant with mRNA expression. Smad2 translocated from cytoplasm to nuclear accumulation in response to TGF-β1, and peaked at 30 min. CONCLUSIONS: Smad2 is present in peritoneal mesothelial cells. TGF-β1 may activate Smad2 expression and translocation to nuclear in a time-dependent and dose-dependent manner. [

关 键 词：腹膜间皮细胞 转化生长因子Β SMAD2 

分 类 号：R363[医药卫生—病理学]