胃癌微转移基因检测的临床应用  

Clinical application of gene detection in micrometastases of gastric cancer

作　　者：刘中宏[1] 李春明[1] 王秀江[1] 龙培滨[1] 

机构地区：[1]哈尔滨市第一医院消化内科,黑龙江省哈尔滨市150010

出　　处：《世界华人消化杂志》2004年第9期2033-2035,共3页World Chinese Journal of Digestology

摘　　要：目的:探讨荧光定量聚合酶链式反应(FQ-PCR)扩增P53基因作为检测胃癌血行微小转移方法的可行性及其表达的临床意义. 方法:应用对胃癌患者转移(n=12)和无转移组(n=18)的外周血的基因进行检测,分析这两组基因的表达与胃癌转移复发之间的关系,并将FQ-PCR与普通PCR的敏感性进行比较. 结果:胃癌患者30例,在转移组中P53基因表达检出为58.3%(7/12);而无转移组中为11.1%(2/18).两组比较,具有显著性差异(P<0.01),表明外周血癌基因表达与转移复发是相关的,其他病种患者外周血中无阳性表达,而28名健康献血员中均为阴性. 结论:P53基因可作为胃癌微小转移的重要指标,FQ-PCR 是检测微小转移的敏感方法,对胃癌的早期诊断和预测微小转移有一定的临床价值.AIM: To analyze feasibility of amplifying P53 gene by FQ-PCR in micrometastasis detection of gastric cancer. METHODS: PCR and FQ-PCR were used to detect the peripheral blood carcinoma gene in the metastased group (n =12) and non-metastased group (n =18) of gastric cancer, to evaluate the correlation between gene expression and micrometastasis in gastric cancer and the sensitivity of PCR and FQ-PCR. RESULTS: P53 expression rate was 58.3% (7/12) in the metastased group, and 11.1% (2/18) in the non-metastased group. There was significant difference between the two groups (P<0.01). CONCLUSION:P53 may be an important marker of micro- metastasis.FQ-PCR can be used to detect micrometastases of gastric cancer for early diagnosis.

关 键 词：微小转移 胃癌患者 临床应用 外周血 FQ-PCR 微转移 P53基因 结论 敏感 显著性差异 

分 类 号：R735.2[医药卫生—肿瘤] R737[医药卫生—临床医学]