肝细胞癌DNA修复酶hMTH1基因mRNA及其蛋白的表达  

Expression of DNA repair enzyme hMTH1 mRNA and its protein in hepatocellular carcinoma

作　　者：周鹤俊[1] 林菊生[1] 周秀敏[1] 黎培员[1] 陶璐薇[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝病研究所,湖北省武汉市430030

出　　处：《世界华人消化杂志》2004年第9期2057-2060,共4页World Chinese Journal of Digestology

摘　　要：目的:通过检测DNA修复酶hMTH1 mRNA及其蛋白在肝细胞癌组织及细胞株中的表达,探讨hMTH1在肝细胞癌发生、发展及防御机制中的作用. 方法:采用半定量逆转录聚合酶链反应(RT-PCR)研究肝癌(HT,n=33)、癌旁组织(HST,n=33)和正常肝细胞株(L-02)、肝癌细胞株(SMMC772 1,HepG2)中hMTH1 mRNA的表达,同时采用免疫组织化学方法原位检测了上述的肝癌组织(n=17)及癌旁组织(n=17)中hMTH1蛋白的表达. 结果:绝大部分检测对象中均有不同程度hMTH1 mRNA及其蛋白的表达.肝癌组织中hMTH1 mRNA的表达较癌旁组织显著升高(t=2.424,P=0.021<0.05);SMMC7721, HepG2细胞中hMTH1 mRNA的表达显著高于L-02细胞(F=6.810,P=0.009<0.01).SMMC7721与HepG2细胞中hMTH1 mRNA的表达没有显著差异(P=0.395>0.05). hMTH1蛋白主要在肝细胞胞质中表达,肝癌组织中hMTH1 蛋白水平明显高于癌旁组织(f=2.618,P=0.019<0.05). 结论:hMTH1mRNA及其蛋白在肝癌组织及肝癌细胞株中表达升高.AIM: To study the expression of DNA repair enzyme hMTH1 mRNA and protein in hepatocellular carcinoma (HCC) tissues, normal liver cell and hepatoma cell lines, and to investigate their function in the progress of HCC. METHODS: Expression of hMTH1 mRNA in matched HCC tissues(33 cases)/surrounding tissues of HCC (33 cases), normal liver cell L-02 and hepatoma cell lines SMMC7721 HepG2 were detected by semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR). hMTH1 protein was detected in corresponding HT tissues (17 cases HT) as well as their surrounding liver tissues (17 cases HST) by immunohistochemistry. RESULTS: The expression level of hMTH1 mRNA in HT was higher than that in HST (t =2.424 , P=0.021<0.05). The expression level of hMTH1 mRNA in two hepatoma cell lines was higher than that in normal liver cell line (F=6.810, P =0.009<0.01). The expression of hMTH1 mRNA in SMMC7721 was similar to that in HepG2. hMTH1 protein was 88.2% (15 of 17) positive in HT and 82.4% (14 of 17) in HST and was mainly distributed in hepatocytesi cytoplasm. The protein level of hMTH1 in HCC tissues was correspondingly higher than in their surrounding tissues (t =2.618, P=0.019<0.05). CONCLUSION: Overexpression of hMTH1 mRNA and its protein in the liver tissues of HCC and hepatoma cell lines is found.

关 键 词：hMTH1 表达 RNA 蛋白 肝癌组织 癌旁组织 SMMC7721 DNA修复酶 基因 细胞 

分 类 号：R735.7[医药卫生—肿瘤] R737[医药卫生—临床医学]