TGF-β1和smad4mRNA在慢性肝炎、肝硬化、肝癌癌旁组织的表达及意义  

Expression of TGF-β1 and smad4 mRNA in chronic hepatitis, liver cirrhosis, hepatocellular carcinoma and para-cancerous tissues, and their significance

作　　者：廖丹[1] 罗光汉[1] 吴继周[1] 江建宁[1] 黄钰全[2] 

机构地区：[1]广西医科大学第一附属医院传染科,广西壮族自治区南宁市530027 [2]广西医科大学第一附属医院病理科,广西壮族自治区南宁市530027

出　　处：《世界华人消化杂志》2004年第9期2091-2094,共4页World Chinese Journal of Digestology

摘　　要：目的:转化生长因子TGF-β1信号转导通路具有重要的细胞调节功能,包括细胞的生长、分化、黏附、转移、细胞外基质的形成及免疫调节等.通过检测慢性病毒性肝炎、肝硬化、肝癌癌旁病理组织TGF-β1和smad4mRNA的表达, 探讨TGF-β/smad通路在肝纤维化形成、发展中的关系及作用机制. 方法:应用免疫组化和原位杂交方法,对照肝组织10例, 肝癌癌旁肝组织17例,慢性病毒性肝炎肝硬化肝穿组织70 例的TGF-β1蛋白和smad4mRNA表达进行检测. 结果:TGF-β1和smad4mRNA在慢性病毒性肝炎中度及肝硬化组织中的表达明显增强,阳性率分别为83.3%,87.0% 和87.5%,87.0%,明显高于对照组(20.0%,20.0%),差异均有显著性(b3P<0.01,)X2=12.3980;b4P<0.01,)X2=14.0609; 和b1P<0.01,X2=14.6953;b2P<0.01,X2=14.0609);而癌旁肝组织二者的表达均低下,与对照组无明显差异;随着肝纤维化的发展TGF-β1和smad4mRNA的表达呈逐渐增强趋势,S3期达高峰,S4期有所回落;二者表达呈正相关(X2=4.5 064,P=0.0336,r=0.2668);肝组织病理可见TGF-β1和smad4mRNA的阳性细胞在肝组织切片上的分布基本一致,大多集中于汇管区、肝窦及纤维条索周边. 结论:TGF-β/smad信号传导通路在肝纤维化的形成和发展中具有重要的促进作用,TGF-β1和smad4mRNA的组织定位检测能准确的评价肝纤维化形成状况及发展趋势.AIM: Transforming growth factor β1 (TGF-β1) signaling pathway is involved in a variety of important cellular regulative functions. including cell growth, differentiation, adhesion migration, extracellular matrix formation and immune regulation. The study was aimed to evaluate the relationship and mechanism of transforming growth factor β1 protein and smad4 mRNA in formation and development of fibrosis by detecting the expression of transforming growth factor β1 protein and smad4 mRNA in liver biopsy of chronic virus hepatitis, hepatocirrhosis and para-cancerous tissues. METHODS: In situ hybridization and immunohistochemis-try were used in 10 cases of normal liver tissue, 17 cases of para-cancerous tissues, 70 cases of chronic virus hepatitis and hepatocirrhosis. RESULTS: Increased expression of TGF-β1 and smad4 mRNA in the intermediate degree of chronic virus hepatitis and hepatocirrhosis; the positive rates of TGF-β1 and smad4 mRNA were obviously higher in the chronic virus hepatitis and hepatocirrhosis (83.3%, 87.0% and 87.5%, 87.0%) than those in the normal liver (20.0%, 20.0%), the expression of TGF-β1 and smad4 mRNA had a significant relationship between the intermediate degree of chronic virus hepatitis, hepatocirrhosis and the normal liver (b3P<0.01, X2=12.3980; b4P<0.01, X2=14.0 609; b1P<0.01, X2 =14.6 953; b2P<0.01, X2=14.0 609); the expression of TGF-β1 and smad4 mRNA in pare-cancerous tissues were significantly lower than that in the chronic virus hepatitis and hepatocirrhosis, as comparied with with the normal liver; the expression of TGF-β1 and smad4 mRNA reached the highest level at the stage S3, and decreased slightly at the stage S4, with positive relevance (X2 =4.5 064, P=0.0336, r=0.2 668), in which most of the positive cells were distributed surroundings of portal tract, central vein, and the sinus. CONCLUSION: TGF-β/smad pathway plays a significant rote in formation and development of hepatic fibrosis, and the localization of TGF-β1 and smad4mRNA in hepatic tissue section can be use

关 键 词：TGF-Β1 SMAD4MRNA 表达 肝癌 肝硬化 癌旁肝组织 对照组 结论 意义 差异 

分 类 号：R735.7[医药卫生—肿瘤] R575[医药卫生—临床医学]