小剂量顺铂联合^(32)磷玻璃微球间质注射抑瘤实验研究  

Study of the Anti-tumor Effects of Interstitial Radiotherapy with ^(32)P Glass Microspheres in Combination with Low Dose Cisplatin on Experimental Mice Solid Tumor S180.

作　　者：李卫国[1] 陈伟良[2] 蒋立坚[1] 吴新中[1] 刘国萍[1] 

机构地区：[1]中山大学附属第三医院口腔科,广东广州510640 [2]中山大学附属第二医院口腔科

出　　处：《广东牙病防治》2004年第4期249-251,共3页Journal of Dental Prevention and Treatment

基　　金：广东省重点攻关基金资助 ;基金号 :2KM0 4 4 0 4S

摘　　要：目的　研究腹腔注射小剂量顺铂对小鼠S180实体瘤3 2 磷玻璃微球间质注射内放射治疗的增敏作用及治疗对瘤细胞分裂周期的影响。方法　将荷S180瘤小鼠随机分为空白对照组、单纯腹腔注射顺铂组、单纯3 2 磷玻璃微球间质注射组和顺铂加3 2 磷玻璃微球内放射联合治疗组 ,分别观察各组肿瘤生长情况 ,2周后取出肿瘤称瘤体质量 ,计算各组抑瘤率 ,瘤体组织行流式细胞仪检查 ,观察各组瘤体细胞周期变化及细胞凋亡率。结果　顺铂组、3 2 磷玻璃微球组及联合组肿瘤抑瘤率分别为 4 7%、4 0 %、76 % ,联合组瘤体质量明显较小 (P <0 .0 5 )。对照组、顺铂组、3 2 磷玻璃微球组、联合组细胞凋亡率分别为 1.36 %、2 1.4 3%、37.6 4 %、6 2 .0 3%。联合组细胞凋亡率明显高于其余 3组 (P <0 .0 0 1)。结论　腹腔注射小剂量顺铂可明显增加3 2 磷玻璃微球间质注射内放射治疗对小鼠S180瘤的抑制作用 ,联合治疗使瘤细胞凋亡率明显提高 。Objective To study the combination effect of low dose cisplatin and 32P-GMS on treating experimental mice solid tumor S180 and to observe apoptosis of the tumor cells. Methods The mice with the solid tumor S180 were divided into control group, DDP group, 32P-GMS group, and combination group (DDP and 32P-GMS) randomly. The weight of tumor was measured separately after two weeks and the cell divide cycle of each group and the rate of apoptosis were detected by FCM. Results The inhibition rates of DDP group, 32P-GMS group and combination group were 47%, 40%, and 76% respectively, and the apoptosis rates of tumor cells were 21.43%, 37.64%, and 62.03% respectively. The apoptosis rates of the experimental groups were significantly higher than that of the control group (P<0.05). The apoptosis rate of combination group was significantly higher than those of DDP group and 32P-GMS group (P<0.001). Conclusion Low dose cisplatin can obviously enhance the inhibiting effect of 32P-GMS. This provides theoretical basis for the clinical use of low dose cisplatin combined with 32P-GMS.

关 键 词：^32磷 顺铂 间质 联合 瘤体 小剂量 细胞凋亡率 玻璃微球 研究 

分 类 号：R737.33[医药卫生—肿瘤] R732.2[医药卫生—临床医学]