三氧化二砷对人肝癌细胞周期相关基因表达的影响  

Expression of cell cycle and associated genes of hepatomacellular carcinoma by arsenic trioxide

作　　者：张霞[1] 王丕龙[1] 陶小红[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,400016

出　　处：《重庆医学》2004年第11期1671-1673,共3页Chongqing medicine

摘　　要：目的　研究三氧化二砷 (As2 O3 )对人肝癌SMMC 772 1细胞周期的阻滞作用及相关基因的表达。探讨As2 O3 抗肝癌作用的机理。方法　体外培养人肝癌细胞株SMMC 772 1,用 2 μmol/LAs2 O3 处理 72h ,流式细胞仪检测细胞周期变化 ;通过免疫细胞化学检测cyclinD1、PCNA基因编码蛋白的表达。结果　As2 O3 使SMMC 772 1细胞周期阻滞在G0 /G1期。与对照组比较 ,经As2 O3 作用的人肝癌细胞cyclinD1、PCNA基因编码蛋白的表达减少。结论　As2 O3 通过干扰细胞周期的进程 ,下调cyclinD1、PCNA基因编码蛋白的表达 ,抑制肿瘤的增殖和转移 ,从而具有抗肿瘤的作用。Objective To study the arrestee of cell cycle and the expression of associated genes by arsenic trioxide. To explore mechanisms of inhibiting HCC by arsenic trioxide.Methods Cultured in vitro, HCC SMMC-7721 were treated for 72h with 2μmol/L arsenic trioxide. The cell cycle was detected by flow cytometry(FCM),the immunohistochemistry was used to detected protein expression of cycline D 1,PCNA. Results SMMC-7721 cell cycle was arrested in G 0/G 1,compared with control, arsenic trioxide induced decrease of the expression of cycline D 1,PCNA in HCC.Conclusion Arsenic trioxide could disturb cell cycle progression of SMMC-7721 and inhibit the HCC through down-regulation expression of cycline D 1,PCNA associated genes.

关 键 词：三氧化二砷 肝细胞癌 细胞周期 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]