松口蘑发酵菌丝体中抗肿瘤糖蛋白的分离纯化及组成分析  

Purification and Component Analysis of Anti-tumor Glycoprotein from Tricholoma Matsutake Sing

作　　者：魏元青[1] 许泓瑜[1] 万彩霞[1] 陶文沂[1] 

机构地区：[1]江南大学生物工程学院生物制药实验室

出　　处：《中国医药工业杂志》2004年第11期650-652,共3页Chinese Journal of Pharmaceuticals

摘　　要：从松口蘑发酵菌丝体中提取得到一种胞内抗肿瘤糖蛋白,经柱色谱纯化得到其主要组分。采用SDS-PAGE法测得其分子量约为26kD,蛋白含量87.49%,糖含量12.51%。组成分析含有17种氨基酸,糖链部分是由5种单糖组成的杂糖链,其组成为阿拉伯糖-木糖-甘露糖-葡萄糖-半乳糖(1.92∶3.38∶15∶2.38∶1,摩尔比)。A glycoprotein was extracted from the mycelia of Tricholoma matsutake and purified by columnchromatography. The molecular weight of the glycoprotein was 26 kD by the SDS-PAGE determination. It contained87.49% protein and 12.51% saccharides. Component analysis dictated that it was composed of 17 kinds of amino acids,and the mol ratio of monosaccharides was Ara∶Xyl∶Man∶Glc∶Gal=1.92∶3.38∶15∶2.38∶1.

关 键 词：松口蘑 抗肿瘤 糖蛋白 组成分析 

分 类 号：R284.2[医药卫生—中药学]