脱氢表雄酮对内皮细胞NO,ET-1生成及ICAM-1表达的影响  

Influence of dehydroepiandrosterone on production of NO, ET-1 and expression of ICAM-1 in endothelial cells

作　　者：周娟[1] 杨予白[1] 奥沛源 雷立权[1] 

机构地区：[1]西安交通大学医学院病理生理学教研室,陕西西安710061 [2]宝鸡市中医医院骨科,陕西宝鸡721001

出　　处：《第四军医大学学报》2004年第20期1843-1845,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :观察脱氢表雄酮对内皮细胞NO合成、ET 1分泌及细胞表面ICAM 1表达的影响 .方法 :培养的人脐静脉内皮细胞系ECV30 4经不同浓度和不同作用时间DHEA处理后 ,采用硝酸还原酶及放免法分别测定培养液中NO及ET 1水平 ,用SABC免疫组化法观察细胞表面ICAM 1的表达 .结果 :与对照组相比 ,随着DHEA浓度的增加 ,培养液中NO的含量从 (387± 12 )降至 (2 4 2± 11) μmol/L (P <0 .0 5 ) ,而ET 1的浓度却从 (397± 13)增至 (6 2 6± 2 9)ng/L (P <0 .0 5 ) .随着时间的延长 ,各对照组培养液中NO含量均无明显变化 (P >0 .0 5 ) ,而DHEA组NO生成量却逐渐降低 (P <0 .0 5 ) ,呈时间依赖 .培养液中ET 1浓度无论是对照组还是DHEA组均随时间的延长而升高 (P <0 .0 5 ) ,但DHEA组升高的更为明显 .无论是增加浓度还是延长时间 ,DHEA均不影响细胞表面I CAM 1的表达 .结论 :DHEA可通过调节内皮细胞NO及ET 1生成量对血管功能进行调控 .AIM: To investigate the influence of dehydroepiandrosterone on the synthesis of NO, secretion of ET-1 and the expression of ICAM-1 in endothelial cells. METHODS: The cultured human umbilical vein endothelial cells ECV304 was treated by DHEA and the level of NO and ET-1 in medium was detected by nitrite/nitrate colorimetric method and radioimmunoassay. The surface expression of ICAM-1 on cells was detected immunohistochemically. RESULTS: In comparison with those of control, the content of NO in medium decreased from (387±12) to (242±11) μmol/L (P<0.05), but the concentration of ET-1 in medium increased from (397±13) to (626±29) ng/L (P< 0.05) along with the increase of concentration of DHEA. The content of NO in medium of control group was similar at every time point(P>0.05), but DHEA time-dependently decreased the content of NO (P<0.05). The concentration of ET-1 in medium time-dependently increased both in control group and in DHEA group (P< 0.05), and it was higher in DHEA group. Higher concentration of DHEA or longer treatment time, DHEA did not affect the ICAM-1 expression. CONCLUSION: DHEA can adjust the function of vessels by regulating the production of NO and ET-1 of endothelial cells.

关 键 词：脱氢表雄酮 ECV304细胞 一氧化氮 内皮缩血管肽-1 胞间粘附分子-1 

分 类 号：R363.2[医药卫生—病理学]