胆汁泡蛋白ELISA检测法的ROC曲线分析  

An analysis of ROC curve in the evaluation of biliary vesicular protein detected by ELISA

作　　者：项建斌[1] 蔡端[1] 方诚[2] 马保金[1] 查锡良[3] 王丽影[3] 张延龄[1] 

机构地区：[1]复旦大学附属华山医院外科,上海200040 [2]复旦大学公共卫生学院 [3]复旦大学基础医学院

出　　处：《中华普通外科杂志》2004年第10期614-616,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金 (3 95 70 695;3 0 0 70 73 7) ;卫生部基金(98 2 12 4)

摘　　要：目的　评价用ELISAkit检测胆固醇性胆结石的准确性。方法　构建胆汁 335 0 0泡蛋白检测试剂盒 ,测定正常人、胆固醇性结石症、胆色素性结石症患者胆囊胆汁泡蛋白含量 ,用受试者工作特征 (receiveroperatingcharacteristic ,ROC)曲线分析评价不同截割点含量的试剂盒诊断价值。结果　胆固醇性结石组、胆色素性结石组和正常人群组胆囊胆汁 335 0 0泡蛋白含量依次为 (2 13± 70 ) μg/ml、(72± 5 5 ) μg/ml及 (6 5± 5 2 ) μg/ml,其中胆固醇性结石组泡蛋白含量明显高于胆色素性结石组和正常人群组 (F =6 0 9,P <0 0 5 ) ;当泡蛋白截割点含量为 14 3μg/ml时 ,该ELISAkit诊断胆固醇性胆石症的敏感度、特异度和符合率分别为 85 %、90 %和 88% ,ROC曲线下面积值高达 0 96。结论　不同人群存在胆汁泡蛋白含量分布的异质性 ,ELISAkit辅助诊断胆固醇性胆结石具有潜在的临床应用价值。Objective To evaluate the diagnostic accuracy of ELISA kit for cholesterol gallstone. Methods The ELISA kit of 33?500 vesicular protein was established by sandwich method, and the concentrations of the protein in gallbladder bile were examined among cholesterol, pigmental gallstone patients and control groups. Results The gallbladder 33?500 vesicular protein (213±70) μg/ml is much higher in cholesterol gallstone patients than in pigmental gallstone patients (72±55) μg/ml and control groups (65±52) μg/ml (F=60.9, P<0.05). When the optimal cut-off point was 143 μg/ml, the sensitivity, specificity and accuracy of the ELISA kit was 85%,90% and 88% for detecting cholesterol gallstone and the maximal area under the ROC curve. Area under the curve of ROC was 0.96. Conclusion 33?500 vesicular protein varies in different groups and ELISA kit helps in establishing the diagnosis of cholesterol gallstone.

关 键 词：胆固醇 胆汁泡蛋白 胆色素 结石症 ELISA 正常人群 ROC曲线分析 胆囊 胆结石 含量 

分 类 号：R575.6[医药卫生—消化系统]