惊厥大鼠海马神经细胞IL-1β和IL-1ra表达及细胞凋亡的研究  

Study of neuronal expression of IL-1β and IL-1ra and neuronal apoptosis in the seizure rats

作　　者：安仁哲[1] 延光海[2] 尹永日[1] 池永学[1] 

机构地区：[1]延边大学医学院附属医院儿科,吉林延边133000 [2]延边大学医学院解剖教研室,吉林延边133000

出　　处：《中风与神经疾病杂志》2004年第5期446-447,共2页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　探讨癫痫发作所致的神经细胞凋亡与白细胞介素 - 1β(IL - 1β)和白细胞介素 - 1受体拮抗剂(IL - 1ra)表达的关系。方法　建立毛果云香碱癫痫动物模型 ,应用原位末端标记法 (TU NEL )和免疫组织化学法 ,检测海马神经细胞凋亡与 IL - 1β和 IL - 1ra表达。结果　癫痫发作后 2 4、4 8h,在大鼠海马 CA1 区 ,TUNEL阳性细胞数、IL - 1β表达和 IL - 1ra表达较对照组显著增高 (P<0 .0 0 1)。 IL - 1β表达与细胞凋亡成正相关 (r=1.0 ,P<0 .0 1)。结论　内源性 IL - 1β和 IL -Objective To study the relationship between neuronal apoptosis and neuronal expression of IL-1β and IL-1ra induced by seizures. Methods Seizures animal model were established by Pilocarpine. Neuronal apoptosis was assessed by in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL). IL-1β and IL-1ra expression were examined by histochemistry. Results TUNEL positive cells and IL-1β and IL-1ra expression were increased in hippocampal CA 1 region at 24 and 48 hours after seizures and there were significant difference compared with controls(P<(0.001)). IL-1β expression were positively correlated with TUNEL positive cells(r=1.0,P<(0.01)). Conclusion Endogeneous IL-1β and IL-1ra may take part in apoptotic process induced by seizures.

关 键 词：IL-1Β IL-1RA 表达 大鼠海马 细胞凋亡 癫痫发作 TUNEL 海马神经细胞 CA1区 白细胞介素-1受体拮抗剂 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R749.16[医药卫生—临床医学]