雌激素硫酸转移酶和BIN1蛋白在乳腺癌组织中的基因表达及其意义  

Expression of Estrogen Sulfotransferase (EST) Gene and Bridging Integrator Protein-1 (BIN1) Gene and Their Significance in Breast Tissues

作　　者：邓文慧[1] 吴宜勇[1] 杨丽[1] 唐蔚青[2] 王抒[2] 

机构地区：[1]卫生部北京医院妇产科,北京100730 [2]卫生部老年病研究所,北京100730

出　　处：《癌症》2004年第11期1302-1305,共4页Chinese Journal of Cancer

摘　　要：背景与目的:雌激素硫酸转移酶(estrogensulfotransferase,EST)是硫酸化代谢雌激素(使雌激素活性降低)的主要酶,BIN1蛋白(bridgingintegratorprotein-1)是一种新发现的具有抑癌特性的c-myc连接蛋白,两者可能在肿瘤发生中起保护作用。本研究检测EST和BIN1基因表达在乳腺癌组织中的改变,探索乳腺癌的发病机制。方法:逆转录多聚酶链式反应(reversetranscriptionpolymerasechainreaction,RT-PCR)方法检测ESTmRNA和BIN1mRNA在人正常乳腺和乳腺癌组织中的表达。结果:ESTmRNA和BIN1mRNA在正常乳腺组织中全部表达,而ESTmRNA在75%乳腺癌组织中表达降低,25%中表达缺失;BIN1在25%乳腺癌组织中表达降低,66.7%中表达缺失。结论:ESTmRNA和BIN1mRNA表达降低或缺失,可能是乳腺癌变的分子机制之一。BACKGROUND &OBJECTIVE: Estrogen sulfotransferase (EST) is an enzyme which metabolizes estrogen into inactive estrogen sulphate. Bridging integrator protein 1 (BIN1) is a novel c myc interacting protein with the features of tumor suppressor. EST and BIN1 may be protective in tumorigenesis. This study was to detect the gene expression of EST and BIN1 in breast cancer tissues,and further investigate the carcinogenesis mechanism of breast cancer. METHODS: The mRNA levels of EST and BIN1 in 12 specimens of normal human breast tissue, and 24 specimens of breast cancer were measured by reverse transcriptase polymerase chain reaction (RT PCR). RESULTS: EST mRNA and BIN1 mRNA were expressed in all normal human breast tissues, while EST mRNA was decreased in 75.0%(18/24), and lost in 25.0%(6/24) breast cancer specimens; BIN1 mRNA was decreased in 25.0%(16/24), and lost in 66.7.0%(16/24) breast cancer specimens. CONCLUSION: Down regulation of EST mRNA and BIN1 mRNA may play an important role in the molecular mechanisms of breast carcinogenesis.

关 键 词：乳腺癌 雌激素硫酸转移酶 BIN1蛋白 

分 类 号：R737.902[医药卫生—肿瘤]