气体信号一氧化氮与硫化氢在肺动脉舒张反应中的相互作用  

The interaction between nitric oxide and hydrogen sulfide on the relaxation reactivity of pulmonary arteries in rats

作　　者：石琳[1] 杜军保[1] 张春雨[1] 闫辉[1] 唐朝枢[2] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院心血管病研究所,北京100034

出　　处：《中国药理学通报》2004年第10期1112-1115,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目 ( 3 0 2 713 73 ) ;教育部博士点基金资助项目 ( 2 0 0 2 0 0 0 10 63 ) ;国家重点基础研究发展规划资助项目 ( 973G2 0 0 0 0 5 690 5 )

摘　　要：目的　探讨气体信号一氧化氮与硫化氢在正常大鼠离体肺动脉舒张反应中的相互作用。方法　正常♂SD大鼠 7只 ,体重 2 5 0～ 2 6 0g ,麻醉后迅速取肺动脉环离体灌注 ,观察它对不同浓度一氧化氮供体硝普钠、硫化氢供体硫氢化钠的舒张反应 ;分别给予血管环DL propargylglycine (PPG)和Nω 硝基 L 精氨酸甲酯 (L NAME) ,观察它们对硝普钠、硫氢化钠舒张反应的影响。结果　肺动脉对不同浓度硝普钠、硫化氢呈现剂量依赖性舒张反应 ,PPG可减弱硝普钠对血管环的舒张作用 ,而L NAME可减弱硫化氢对血管环的舒张作用。结论　硫化氢可以独立及与一氧化氮协同发挥舒张血管效应 ,硝普钠可以独立及与硫化氢协同发挥舒张血管效应 ,气体信号分子网络在肺血管舒张反应的机制中占据重要地位。Aim To explore the interaction between nitric oxide( NO) and hydrogen sulfide(H 2S) on the relaxation reactivity of pulmonary arteries in rats.Methods Seven male healthy rats were anaesthesed with chloral hydrate; the pulmonary artery of each rat was removed for the study. Th e reactivities of pulmonary artery rings in response to different doses of NO do nar-sodium nitroprusside(SNP) and H 2S donar-NaHS were measured in vitro.DL-propargylglycine(PPG)and Nω-nitor-L-methyl ester(L-NAME) were provided to pulmonary artery, respectively;the relaxation reactivities of pulmonary artery were observed.Results The relaxation reactivities of pulmonary arteries showed a dose-dependent increase in response to different doses of SNP and H 2S.The relaxation reactivity to SNP decreased by PPG. The relaxation reactivity to H 2S decreased by L-NAME.Conclusion H 2S acted as a vasorelaxator either independently or accompanied with NO, SNP acted as a vasorelaxator either independently or accompanied with H 2S;the networ k of gastransmitter played an important role in the relaxation of pulmonary arte ries.

关 键 词：肺动脉 硫化氢 硝普钠 舒张 

分 类 号：R-332[医药卫生] R322.121