盐酸小檗碱致大鼠肝脏代谢相关基因表达变化的基因芯片研究  

Study on differential expression of genes associated with metabolism by cDNA microarry in rat liver treated with berberine chloride

作　　者：辛华雯[1] 吴笑春[1] 李罄[1] 余爱荣[1] 张勤[1] 仲明远[1] 朱敏[1] 刘幼英[1] 

机构地区：[1]广州军区武汉总医院临床药理科,湖北武汉430070

出　　处：《中国药理学通报》2004年第10期1122-1126,共5页Chinese Pharmacological Bulletin

基　　金：湖北省自然科学基金资助项目 ( 2 0 0 2AB114 )

摘　　要：目的　研究小檗碱对大鼠肝脏基因表达谱的影响。方法　大鼠予以盐酸小檗碱灌胃 6d ,摘取肝脏提取总RNA。用Cy3和Cy5两种荧光物质分别标记阴性对照组和盐酸小檗碱组肝组织cDNA ,制备成探针与表达谱芯片进行杂交 ,获得的荧光信号图像用计算机扫描后进行数据分析 ,计算每点的Cy5和Cy3信号强度比值。结果　在被检测的 4 0 96个基因中 ,盐酸小檗碱组差异表达基因 2 36个 ,与代谢相关的差异表达基因共 32个 ,其中表达上调的代谢相关基因 8个 ,包括大鼠环核苷酸磷酸二酯酶、异柠檬酸脱氢酶 3、心肌黄酶和葡萄糖 6 磷酸酶等。表达下调的代谢相关基因 2 4个 ,包括尿苷二磷酸 葡糖醛酰转移酶、细胞色素P4 5 0 2E1、谷胱甘肽S 转移酶 1、16 α 碳腈孕烯醇酮诱导的细胞色素P4 5 0酶和S 腺苷 L 甲硫氨酸脱羧酶等。结论　小檗碱的降血糖。Aim To study the effects of berberine chloride(Ber) on ge ne expression in rat liver. Methods After orally treated with berberi ne chloride for 6 days, the rats were sacrificed and the liver was taken out to extract total RNA. cDNA from negative control group and Ber group were labeled with Cy3-dCTP and Cy5-dCTP separately. These probes were hybridized with cDNA microarray. The acquired image was scanned and analyzed by GenePix Pro 3.0 soft ware. The intensity ratio of Cy5 to Cy3 about each spot was calculated. Re sults Among 4096 genes, 236 genes exhibited differential expression in the group treated with Ber.32 genes related to cell metabolism were differentially expressed with 8 genes up-regulated and 24 genes down-regulated. The genes up -regulated included 2′,3′-cyclic nucleotide 3′-phosphodiesterase gene, is ocitrate dehydrogenase 3 gene, diaphorase gene and glucose-6-phosphatase gene. The genes down-regulated included UDP-glucuronosyltransferase gene,cytochrom e P450 2E1 gene, glutathione S-transferase 1 gene ,pregnenolone 16-alpha-car bonitrile-inducible cytochrome P450 gene and S-adenosyl-L-methionine decarbo xylase gene. Conclusion The antihyperglycaemic activity ,anti -cancer effects of Ber and drug interactions between Ber and other drugs maybe related with differential expression of some genes associated with cell metaboli sm.

关 键 词：盐酸小檗碱 肝脏 代谢酶 基因芯片 

分 类 号：R-332[医药卫生] R282.71