小檗胺对多药耐药K562/Adr细胞作用的研究  

Study on Effect of Berbamine on Multidrug Resistance Leukemia K562/Adr Cells

作　　者：董庆华[1] 郑树[1] 徐荣臻[1] 吕庆华[1] 何立明[1] 

机构地区：[1]浙江大学附属第二医院肿瘤研究所,杭州310009

出　　处：《中国中西医结合杂志》2004年第9期820-822,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省科技厅资助课题 (No.0 2 1 1 0 82 1 8- 4 );浙江省中医药管理局资助课题 (No.2 0 0 2Y0 0 6)

摘　　要：目的研究小檗胺诱导人白血病K5 6 2 /Adr细胞凋亡及逆转多药耐药的作用及机理。方法采用MTT法测IC50 值 ,流式细胞仪AnnexinVFITC PI法检测细胞凋亡发生率 ,PI染色法检测凋亡峰及细胞周期 ,同时以FCM检测Caspase 3、P GP蛋白表达及细胞内药物积聚能力 ,RT PCR法检测mdr 1基因表达。结果小檗胺能抑制人白血病K5 6 2 /Adr细胞生长且呈剂量依赖关系 ,并能诱导细胞凋亡 ,使Caspase 3蛋白表达及细胞药物外排能力增加 ,同时降低mdr 1基因mRNA和蛋白表达水平。结论小檗胺能激活Cas pase 3以诱导人白血病K5 6 2 /Adr细胞凋亡 ,同时能通过降低mdrObjective To study the effect and mechanism of berbamine on the apoptosis of multidrug resistant leukemia K562/Adr cells and in reversing the drug resistance. Methods IC 50 value of K562/Adr cell was determined with MTT method, cell apoptosis rate was analyzed by flow cytometry with Annexin V FITC PI assay, with the peak and cell cycle detected by PI staining. At the same time, flow cytometry was also used in determining Caspase 3, P GP protein expression and drug accumulating capacity in cells, and RT PCR method was used to analyze the gene expression of mdr 1. Results Berbamine could inhibit human leukemia K562/Adr cell growth in dose dependent manner, it could also induce cell apoptosis, increase the protein expression of Caspase 3 and the drug excretion capacity of cells, reduce the mRNA and protein expression levels of mdr 1 gene. Conclusion Berbamine could activate Caspase 3 to induce human leukemia K562/Adr cell apoptosis, and by reducing mdr 1 gene expression to reverse its multidrug resistance.

关 键 词：K562/ADR 小檗胺 多药耐药 细胞凋亡 表达 白血病 诱导 细胞作用 能力 结论 

分 类 号：R733.7[医药卫生—肿瘤]