靶向性基因导入系统介导p16基因对喉鳞状细胞癌抑制作用  被引量：1

e inhibition of laryngeal cancer cell (Hep-2) by p16 gene mediated with non - viral GE7 polypeptide delivery system</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=10855562' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('gt7IUc6pIY6nm9f5yW2slEAqzFSeD76pXL2JuNjb3+4=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="gt7IUc6pIY6nm9f5yW2slEAqzFSeD76pXL2JuNjb3+4="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" 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title='阎淑惠[1]'>阎淑惠[1]</a> <a href='/writer/article.aspx?id=100000004105003' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e7%8e%8b%e6%88%90%e5%85%83%5d' title='王成元[1]'>王成元[1]</a> <a href='/writer/article.aspx?id=100000007738358' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e7%94%b0%e5%9f%b9%e5%9d%a4%5d' title='田培坤[2]'>田培坤[2]</a> <a href='/writer/article.aspx?id=100000004393927' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%a1%be%e5%81%a5%e4%ba%ba%5d' title='顾健人[2]'>顾健人[2]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=789&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e5%8c%97%e4%ba%ac%e5%a4%a7%e5%ad%a6%e7%ac%ac%e4%b8%80%e5%8c%bb%e9%99%a2%e8%80%b3%e9%bc%bb%e5%92%bd%e5%96%89%e5%a4%b4%e9%a2%88%e5%a4%96%e7%a7%91' title='[1]北京大学第一医院耳鼻咽喉头颈外科,北京100034'>[1]北京大学第一医院耳鼻咽喉头颈外科,北京100034</a> <a href='/organ/article.aspx?id=1773&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e4%b8%8a%e6%b5%b7%e5%b8%82%e8%82%bf%e7%98%a4%e7%a0%94%e7%a9%b6%e6%89%80%e7%99%8c%e5%9f%ba%e5%9b%a0%e5%92%8c%e7%9b%b8%e5%85%b3%e5%9f%ba%e5%9b%a0%e5%9b%bd%e5%ae%b6%e9%87%8d%e7%82%b9%e5%ae%9e%e9%aa%8c%e5%ae%a4' title='[2]上海市肿瘤研究所癌基因和相关基因国家重点实验室,上海200032'>[2]上海市肿瘤研究所癌基因和相关基因国家重点实验室,上海200032</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=98275A' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e4%b8%ad%e5%9b%bd%e8%80%b3%e9%bc%bb%e5%92%bd%e5%96%89%e5%a4%b4%e9%a2%88%e5%a4%96%e7%a7%91%5d' title='中国耳鼻咽喉头颈外科'>《中国耳鼻咽喉头颈外科》</a>2004年第4期249-252,共4页<em>Chinese Archives of Otolaryngology-Head and Neck Surgery</em></p><p class='fund'><strong>基　　金：</strong>卫生部科学研究基金(98-2-228)</p><p class='abstrack'><strong>摘　　要：</strong>目的 探讨一种新的靶向性非病毒型基因导入系统GE7-HA20介导P16基因对喉鳞状细胞癌的抑制作用。方法 建立喉癌裸鼠移植瘤模型;构建外源性基因(β-半乳糖苷酶报道基因及P16基因)和多肽载体系统复合物;在体内外观察P16基因对喉癌的抑制情况;LSAB法观察Hep-2细胞转染前后的P16表达。结果 靶向性多肽基因导入系统可将β-gal导入裸鼠移植瘤内呈阳性反应呈蓝染,LSAB显示转染后P16基因的表达明显升高,P16基因具有抑制肿瘤生长作用并有统计学意义(P<0.05)。结论 GE7-HA20系统借助于表皮生长因子受体(epidermal growth factorreceptor,EGFR)可靶向性将目的基因导入喉癌中并得到高效表达;体内外导入p16基因后可显著抑制喉鳞状细胞癌。<em><abstract>JECTIVE To investigate the inhibition of laryngeal cancer cell (Hep - 2) by p16 gene mediated with non - viral GE7 polypeptide delivery system. METHODS A complex of β-galactosidase (β-gal) or p16 gene and gene delivery system was prepared, then inoculated into implanted tumor in nude mice and Hep - 2 cell. The anti - tumor effect were evaluated both in vivo and in vitro study. Histological examination was performed on implanted tumors. LSAB method was used to determine the expression of p16 . RESULTS Positive blue staining was noticed after β-gal transfered 12 hrs. p16 gene could inhibit the growth of laryngeal cancer cell. LSAB demonstrated over - expression of p16 after transfection. Statistic analysis showed that there was a significant difference between p16 group and the control group (P < 0.05). CONCLUSION A non - viral GE7 polypeptide gene delivery system could transfer exogenous genes into laryngeal cancer cell with high efficiency and targetability. p16 gene can inhibit tumor growth in vivo and vitro.This study also further support that GE7 would be applicable as a new targeted, high efficient gene delivery system to the gene therapy of squamous cell carcinoma of head and neck.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=7589948' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5bP16%e5%9f%ba%e5%9b%a0%5d' title='P16基因'>P16基因</a> <a href='/subject/article.aspx?id=7353162' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e9%9d%b6%e5%90%91%e6%80%a7%5d' title='靶向性'>靶向性</a> <a href='/subject/article.aspx?id=6508204' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%96%89%e9%b3%9e%e7%8a%b6%e7%bb%86%e8%83%9e%e7%99%8c%5d' title='喉鳞状细胞癌'>喉鳞状细胞癌</a> <a href='/zk/search.aspx?key=K%3d%5b%e5%96%89%e7%99%8c%5d' title='喉癌'>喉癌</a> <a href='/subject/article.aspx?id=4931313' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e4%bb%8b%e5%af%bc%5d' title='介导'>介导</a> <a href='/subject/article.aspx?id=4577959' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%a3%b8%e9%bc%a0%e7%a7%bb%e6%a4%8d%e7%98%a4%5d' title='裸鼠移植瘤'>裸鼠移植瘤</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%8a%91%e5%88%b6%e4%bd%9c%e7%94%a8%5d' title='抑制作用'>抑制作用</a> <a href='/zk/search.aspx?key=K%3d%5b%e4%bd%93%e5%86%85%5d' title='体内'>体内</a> <a href='/zk/search.aspx?key=K%3d%5bHA%5d' title='HA'>HA</a> <a href='/zk/search.aspx?key=K%3d%5b%e8%bd%ac%e6%9f%93%5d' title='转染'>转染</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR739.65' title='R739.65'>R739.65</a>[<a href = '/domain/article.aspx?id=503' >医药卫生—肿瘤</a>] <a href='/zk/search.aspx?key=C%3dR735' 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