转染弓形虫致密颗粒蛋白-1编码基因的小鼠巨噬细胞生物学特性  

Biological Features of Mouse Macrophage Transfected with Toxoplasma gondii GRA-1 Gene

作　　者：彭碧文[1] 林建银[1] 章涛[1] 蒋明森[2] 

机构地区：[1]福建医科大学分子医学研究中心,福州350004 [2]武汉大学医学院,武汉430071

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第5期283-286,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家教育部重点项目 (No .0 2 0 74);福建省教育厅重点项目(No.JA0 2 2 1 6);福建省科技三项费用(No.2 0 0 1 0 72 )~~

摘　　要：目的　探讨转染弓形虫致密颗粒蛋白 1编码基因 (P2 4)前后巨噬细胞生物学性状的改变。　方法　将构建的重组质粒用脂质体介导 ,转染到小鼠巨噬细胞RAW 2 64 .7,抗生素G418筛选出阳性克隆 ,逆转录 聚合酶链反应方法鉴定并比较不同细胞株P2 4的表达。光学显微镜观察形态学改变并绘制生长曲线。　结果　Cyto P2 4 RAW 2 64 .7、Nuc P2 4 RAW 2 64 .7及Mito P2 4 RAW 2 64 .7三株细胞的P2 4mRNA表达水平无显著差异 ,ER P2 4 RAW 2 64 .7明显高于前三者。而转染空载体的 4株细胞未见P2 4 mRNA的表达。ER P2 4 RAW2 64 .7较未转染的细胞贴壁速度快、生长能力强。　结论　P2Objective To investigate the change of biological features of macrophages after transfected by Toxoplasma gondii GRA 1 genes (P24). Methods The transfected cells Cyto P24 RAW264.7, Nuc P24 RAW264.7, Mito P24 RAW264.7 and ER P24 RAW264.7 were studied by RT PCR to determine the P24 mRNA expression. Growth features of the cells were examined with microscopy and the cell growth curve was developed. Results In four cell lines, expression of ER P24 RAW 264.7 was found to be higher than the other three, and there was no P24 mRNA expression in either of the cells without P24 insert. The attachment and the proliferation of ER-P24-RAW264.7 were more rapid than normal RAW264.7. Conclusion Transfection of mouse macrophages ER RAW264.7 strain with T. gondii P24 gene leads to a prominent change of biological features in the studied cell line.

关 键 词：转染 巨噬细胞 RAW264.7 弓形虫 编码基因 小鼠 蛋白 生长 生物学特性 生物学性状 

分 类 号：R735[医药卫生—肿瘤] R737[医药卫生—临床医学]