日本血吸虫线粒体DNA两个分子的遗传变异  

Studies on the Genetic Variation of Two Mitochondrial DNA Molecules of Schistosoma japonicum

作　　者：郭凯文[1] 牛安欧[1] 

机构地区：[1]华中科技大学同济医学院病原生物学系,武汉430030

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第5期300-302,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：湖北省血吸虫病地方病防治办公室资助 (No.2 0 0 2 0 3)~~

摘　　要：目的　从线粒体DNA的 2个分子 ,探讨我国日本血吸虫的遗传变异。　方法　试剂盒抽提基因组总DNA后 ,以特异性引物对线粒体还原型烟酰胺腺嘌呤二核苷酸 (NADH)脱氢酶 1(ND1)和细胞色素c氧化酶I(COI)进行PCR扩增 ,将PCR产物分别测序 ,并以生物信息学方法加以比较 ,构建系统进化树。　结果　序列系统进化树显示日本血吸虫中国大陆株与中国台湾株之间差异较大 ,在树状图中可归为 2类 ;中国大陆山区型地域株 ,即云南洱源和四川天全在树状图中归为 1类 ;中国大陆湖沼洲滩型地域株 ,即湖南岳阳、江西新建和安徽贵池 3个地域株在树状图中处于并列位置 ;湖北省境内不同地域株在树状图中归为 1类。　结论　我国各地日本血吸虫存在不同程度的遗传变异 ,各地域株间亲缘关系密切 。Objective To study the variation of Schistosoma japonicum through two mitochondrial DNA molecules. Methods Genomic DNA was isolated with kit, and the mitochondrial NADH dehydrogenase 1(ND1) and cytochrome c oxidase I (COI) gene fragments were amplified by polymerase chain reaction(PCR) and sequenced. The gene trees were constructed and the acquired data were analyzed with the help of bioinfotmatics. Results The gene trees showed that the Taiwan isolate and the mainland isolates can be divided in two groups: a group from the hilly region (Yunnan and Sichuan), another group from the lake region (Hunan, Jiangxi and Anhui); isolates from Hubei are at a different position on the gene trees. Conclusion There are variations among the geographic isolates of Schistosoma japonicum in China, nevertheless, they have close kinship.

关 键 词：地域株 日本血吸虫中国大陆株 线粒体DNA ND1 还原型烟酰胺腺嘌呤二核苷酸 NADH 细胞色素C氧化酶 遗传变异 树状图 总DNA 

分 类 号：R383[医药卫生—医学寄生虫学] S567.31[医药卫生—基础医学]