黏着斑激酶在大肠上皮不同病变中表达及意义的研究  

Expression of focal adhesion kinase protein in colonic different lesions and clinical significance

作　　者：李楠[1] 顾凯华[1] 朱建华[1] 范志忠[2] 李宁[2] 

机构地区：[1]解放军309医院消化科,北京100091 [2]解放军309医院病理科,北京100091

出　　处：《肿瘤防治杂志》2004年第10期1057-1060,共4页China Journal of Cancer Prevention and Treatment

摘　　要：目的 :探讨黏着斑激酶(focaladhesionkinase ,FAK )在大肠上皮不同病变中的表达及临床病理参数的相关性。方法 :应用免疫组织化学SABC法 ,分析 49例结直肠癌、2 3例腺瘤、10例非肿瘤型息肉。结果 :49例大肠癌中FAK表达 4例 ( 84 4% ) ,FAK的高表达与大肠癌的Duke’s分期、浆膜浸润、淋巴结转移均呈正相关。 2 3例腺瘤标本中 ,FAK表达水平相一致。结论 :大肠癌的侵袭转移与相关基因协同作用密切相关 ,在大肠癌的侵袭转移中 ,FAK的表达具有正负调节的协同作用可能 。OBJECTIVE:To explore the role of the FAK expression in human colonic different lesions and normal tissues,and the correlation between the expression of FAK and clinicopathological data including tumor differentiation,Duke’s stage,etc. METHODS: By using SP immunohistochemical techniques combined with image,the expression of FAK was detected and analysized in 49 colonic carcinoma, 23 adenoma and 10 polypoid lesions.RESULTS: The expression of FAK was weak in most benign colonic epithelium but was up-regulated at moderate or strong levels in invasive colon carcinoma.The immunohistochemical analyses revealed a correlation between tumor stage and the level of FAK, especilly positively expressed on vascular endothelium and lymphatic endothelium of adjacent infiltration carcinoma. The staining of FAK was higher in low differentiation cancer cell than in well differentiation. The cancer with metastasis had more FAK protein than the cancers without metastasis.but significantly related to Dock’s stage.but not related to tumor size. CONCLUTIONS:FAK significantly overexpresses in colon carcinoma, implying that FAK may play an important role in colon carcinoma or colon carcinoma progression.FAK protein expression level in colon cancer tissues may sever as a new parameter for judging tumor biological behavior and judging prognosis.

关 键 词：结肠直肠肿瘤/酶学 结直肠肿瘤/病理学 肿瘤浸润 蛋白质酪氨酸激酶/分析 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]