用酶联免疫法测定水产品中氯霉素的残留量  

Enzyme Linked Immunosorbent Assay(ELISA) for Quantitative Analysis of Chloramphenicol Residues in Aquatic Products

作　　者：沈美芳 赵文亚[2] 费志良 吴光红 耿雪冰 

机构地区：[1]江苏省水产质量检测中心,南京210017 [2]南京农业大学食品科技学院,南京210095

出　　处：《南京师范大学学报（工程技术版）》2003年第2期1-4,共4页Journal of Nanjing Normal University(Engineering and Technology Edition)

基　　金：江苏省"十五"科技攻关项目 (BE2 0 0 13 85 );江苏省科技厅社会公益项目 (BM2 0 0 170 4)资助

摘　　要：运用酶联免疫法测定水产品中的氯霉素残留 .氯霉素浓度在 0 0 5～ 4 0 5 μg/kg范围内呈线性 ,线性方程为Y =0 3 12 11-0 3 40 14X ,r=0 994.向样品中分别添加 0 45、1 3 5、4 0 5 μg/kg 3个浓度水平的氯霉素 ,平均回收率分别为84 5 %、88 4%和 79 9% ,最低检测限为 90ng/kg ,批内变异系数为 6 0 %～ 8 5 % ,批间变异系数为 5 8%～ 14 3 % .结果表明该方法灵敏度高 ,重复性好 。Chloramphenicol residues in aquatic products were analyzed by ELISA. The calibration curve was linear in the range of 0 05～4 05?μg/kg. The average recoveries were 84 5%?88 4% and 79 9% respectively when samples were spiked with 0 45?1 35 and 4 05?μg/kg. The detection limit was 90?ng/kg. The intra assay coefficients of variation were 6 0%～8 5% and inter assay cofficients of variation were 5 8%～14 3%.The method is sensitive and suitable for the determination of trace chloramphenicol in aquatic products.

关 键 词：水产品 氯霉素 酶联免疫法 

分 类 号：R978.13[医药卫生—药品] TQ460.72[医药卫生—药学]