Akt信号通路在左归丸抑制左旋单钠谷氨酸大鼠神经细胞凋亡中的作用  

Role of Akt signal pathway in inhibition of neuronal apoptosis by Chinese medicine Zuogui pill in adult rats treated with monosodium glutamate

作　　者：高唱[1] 王景周[1] 吴伟康[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所神经内科,重庆400042 [2]中山大学中山医学院中西医结合研究所,广东广州510080

出　　处：《中国病理生理杂志》2004年第11期2016-2019,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .30 2 716 5 2 )

摘　　要：目的 :观察Akt信号通路在左旋单钠谷氨酸 (MSG)和左归丸 (ZGW)对成年大鼠神经细胞凋亡中的作用。方法 :采用流式细胞仪和Westernblot方法检测细胞凋亡率和Akt信号通路变化。结果 :MSG可增加细胞凋亡率 ,显著降低Akt(Ser4 73)和Akt(Thr30 8)水平 ;明显增加FKHR(Ser2 5 6 )、GSK - 3β(Ser9)和PTEN表达。ZGW可抑制MSG的部分效应。结论 :左归丸能抑制MSG大鼠神经细胞凋亡 。AIM: To explore the role of Akt signal pathway in apoptosis of neural cells in adult rats treated with Zuogui Pill, a Chinese medicine. METHODS: Flowcytometry and Western blotting methods were used to investigate the changes of cellular apoptosis rate and Akt signal pathway. RESULTS: Monosodium glutamate (MSG) could increase cellular apoptosis rate and significantly restrained the phosphorylation of Akt (Ser473) and Akt (Thr308), and markedly increased the levels of phospho-FKHR (ser256), GSK-3β (Ser9) and PTEN. Zuogui Pill partly inhibited the above effects of MSG. CONCLUSION: Zuogui pill effectively inhibits the neural apoptosis induced by MSG, and Akt pathway is involved in the neuronal protection of Zuogui pill. [

关 键 词：左归丸 细胞凋亡 AKT信号通路 

分 类 号：R363[医药卫生—病理学]