芥子气中毒后肺组织内皮素-1及内皮素A受体的含量和转录表达的研究  

Transcription and expression of endothelin-1 and endothelin A receptor in lung after mustard intoxication

作　　者：曹霞[1] 张黎明[1] 陈志龙[1] 李正银[1] 辜清[2] 蔡芸[1] 

机构地区：[1]第二军医大学海军医学系毒物药物教研室,上海200433 [2]南昌大学生命科学与食品工程学院,江西南昌330047

出　　处：《中国病理生理杂志》2004年第11期2024-2027,共4页Chinese Journal of Pathophysiology

基　　金：全军医药卫生"十五"重点 (指令 )课题基金资助项目 (0 1L0 5 4 )

摘　　要：目的 :探讨内皮素 - 1(ET - 1)、内皮素A受体 (ETAR)在芥子气中毒病理生理过程中的作用和地位。方法 :应用免疫组化及RT -PCR技术 ,观测大鼠芥子气中毒后肺组织ET - 1、ETAR的转录表达变化 ,相互关系及与组织损伤的关系。结果 :中毒后肺组织ET - 1的含量明显高于对照组 ,2h达峰值 ,但在 12h后低于对照组 ;中毒后肺组织ET - 1及ETAR的mRNA表达均高于对照组 ,ET - 1mRNA在 2 4h仍高于正常 ,ETARmRNA在 2 4h低于正常组。结论 :ET -AIM: To investigate the role of endothelin-1 (ET-1), endothelin A receptor (ETAR) in pathophysiology of the lung after mustard intoxication. METHODS: The changes of transcription and expression of ET-1 and ETAR in the lung and their relationship with the tissue damage were studied with immunohistochemistry and RT-PCR. RESULTS: The level of ET-1 in the lung was significantly increased at 2 h, but decreased at 12 h after mustard intoxication. The transcription and expressin of ET-1 and ETAR were significantly increased at the early stage after intoxication. mRNA of endothelin-1 was increased, while mRNA of endothelin A receptor was down-regulated after (12 h.) CONCLUSION: ET-1 and ETAR in lung may play important roles in the pathophysiological process of mustard intoxication. [

关 键 词：芥子气 内皮缩血管肽1 受体 内皮缩血管肽 肺 

分 类 号：R363[医药卫生—病理学]