雷公藤内酯醇对大鼠哮喘模型肺组织IL-13表达的影响  

Effect of triptolide on expression of interleukin-13 in lung tissue of rat asthmatic model

作　　者：张晓云[1] 张焕萍[1] 杜永成[1] 许建英[1] 

机构地区：[1]山西医科大学第一医院呼吸内科,山西太原030001

出　　处：《中国病理生理杂志》2004年第11期2041-2044,共4页Chinese Journal of Pathophysiology

基　　金：山西省科技发展计划资助项目 (No .972 171)

摘　　要：目的 :探讨白细胞介素 13(IL - 13)在大鼠哮喘模型肺组织中的表达以及雷公藤内酯醇对IL - 13表达的影响。方法 :建立大鼠哮喘模型 ,苏木素 -伊红染色观察肺组织炎症改变 ;逆转录 -聚合酶链反应 (reversetran scriptionpolymerasechainreaction ,RT -PCR)对IL - 13mRNA表达进行半定量 ;用酶联免疫吸附试验 (enzyme -linked -immuno -sorbentassay ,ELISA)检测肺组织匀浆细胞因子IL - 13水平。结果 :哮喘组肺组织嗜酸性粒细胞 (EOS)计数、肺组织IL - 13/GAPDHmRNA以及IL - 13蛋白质水平明显高于对照组 ,均有显著性差异 (P <0 0 5 )。雷公藤内酯醇治疗组EOS计数与正常对照组之间差异无统计学意义 (P >0 0 5 ) ,IL - 13/GAPDHmRNA以及IL - 13蛋白质水平明显低于哮喘组 ,但仍高于对照组 ,均有显著性差异 (P <0 0 5 )。结论 :IL - 13的高表达参与了哮喘的病理生理过程 ;雷公藤内酯醇对IL - 13的高表达有部分抑制作用。提示抑制IL -AIM: To investigate the expression of interleukin-13 (IL-13) in lung tissue of rat asthmatic model and effect of triptolide on expression of IL-13. METHODS: A asthmatic model was established and inflammation changes of lung tissues were examined by haematoxylin-eosin staining. Reverse transcriptio polymerase chain reaction (RT-PCR) was used to semiquantitate the expression of IL-13 mRNA, and enzyme-linked-immuno-sorbent assay (ELISA) was used to detect protein level of IL-13. RESULTS: There was a significant increase in the count of EOS, the expression of IL-13 mRNA and IL-13 protein level in lung tissue of asthmatic rats compared with those of triptolide-treated rats (P<0.05, respectively). There was no statistics difference in EOS count between triptolide-treated rats and control (P>0.05). The expression of IL-13 mRNA and IL-13 protein level in triptolide-treated rats were significant lower than that of asthmatic rats (P<0.05, respectively), yet higher than that of control (P<0.05, respectively). CONCLUSION: The overexpression of IL-13 mRNA and higher protein level of IL-13 in asthmatic rats is involved in the pathophysiological process of asthma development, and triptolide inhibits the asthma inflammation, at least partly, via inhibiting IL-13 overexpression. [

关 键 词：哮喘 白细胞介素13 气道炎症 雷公藤内酯醇 

分 类 号：R363[医药卫生—病理学]