急性胰腺炎大鼠腹水白细胞cPKC-α水平的变化及汉防己甲素等的影响  

Change of cPKC-α level in the ascitic white blood cells of AP rats and effects of tetrandrine and aspirin

作　　者：李丽娟[1] 李永渝[2] 张红[2] 黄平[1] 

机构地区：[1]贵州省遵义医学院病理生理教研室,贵州遵义563003 [2]上海同济大学医学院病理生理教研室,上海200092

出　　处：《中国病理生理杂志》2004年第11期2063-2066,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (NO .30 370 6 4 3)

摘　　要：目的 :观察急性胰腺炎 (AP)大鼠腹水白细胞传统型蛋白激酶C -α(cPKC -α)的水平变化及汉防己甲素 (Tet)等药物的影响 ,并对其机制进行探讨。方法 :取 5 6只SD大鼠随机分为 4组 :假手术组 (SO +NS组 ) ,AP对照组 (AP +NS组 ) ,AP +阿司匹林 (ASP)组 ,AP +Tet组 ,采用牛磺脱氧胆酸钠复制AP模型 ,模型制成后 10min ,AP +Tet组腹腔注入Tet(80mg/kg·BW) ;AP +ASP组经食管灌入ASP混悬液 (12 5mg/kg·BW )。各组动物在相应处理后1h、5h ,提取腹水白细胞 ,制备白细胞裂解液 ,用免疫印迹 (Westernblot)和化学发光法检测其cPKC -α水平 ;同时分别取动物血浆测定淀粉酶 (AMY)活性 ;取 5h大鼠胰腺组织作病理检查。结果 :ASP和Tet组胰腺病理损伤轻于AP +NS组 ,血浆AMY活性低于AP +NS组 ,腹水白细胞cPKC -α水平高于AP +NS组 (P <0 0 5或P <0 0 1)。结论 :ASP、Tet可增加AP大鼠腹水白细胞cPKC -α的水平 ;ASP。AIM: To investigate the alteration of classical protein kinase C-α(cPKC-α)in the ascitic white blood cells of rats with acute pancreatitis (AP), and explore the effects of aspirin (ASP) or tetrandrine (Tet) on them. METHODS: The total of 56 health SD rats were divided into four groups, AP+ASP group, AP+Tet group, AP+normal saline (NS) group, and sham operation control (SO) group. AP model was induced by a retrograde injection of 3% sodium deoxycholate into the pancreatic duct. The AP+Tet group received a intraperitoneal injection of Tet (80 (mg/kg)), respectively. The AP+ASP group received an infusion of ASP (12.5 mg/100 g) by use of a nose-gastric catheter. At 1 h, 5 h after the treatment, cPKC-α in the ascitic white blood cells of AP rats was measured by Western blot and chemiluminescence, and the semi-quantitative values was obtained by Gel-pro analyzer. RESULTS: The values of cPKC-α decreased in AP+NS group, but increased significantly in the groups treated with ASP and Tet (P<0.05, or P<0.01). The levels of AMY increased in AP+NS group and decreased in the treated groups significantly (P<0.05, or P<0.01). The morphological lesions in the pancreas were attenuated by these medicines. CONCLUSIONS: 1. ASP and Tet increase cPKC-α levels in the ascitic white blood cells of rats with acute pancreatitis. 2. ASP and Tet have protective effects on the morphology and function of the pancreas in the course of AP. [

关 键 词：胰腺炎 蛋白激酶C 阿司匹林 汉防己甲素 大鼠 

分 类 号：R363[医药卫生—病理学]