碱性成纤维细胞生长因子对大鼠缺血心肌的保护作用  

Protective effects of basic fibroblast growth factor against myocardial ischemia in rats

作　　者：芦丽莉[1] 陈吉田[2] 王丹峰[2] 刘艳波[1] 葛贺[1] 孔繁利[1] 刘自强[1] 

机构地区：[1]北华大学医学院病理生理学教研室,吉林吉林132011 [2]北华大学医学院附属医院,吉林吉林132011

出　　处：《中国病理生理杂志》2004年第11期2098-2100,共3页Chinese Journal of Pathophysiology

基　　金：吉林市科委资助项目 (No.2 0 0 10 35 )

摘　　要：目的 :观察碱性成纤维细胞生长因子对大鼠缺血心肌的保护作用 ,并探讨其作用机制。方法 :实验动物分为 (1)假手术组 (shamcontrolgroup) ;(2 )缺血 +生理盐水组 (ischemia +salinegroup) ;(3)缺血 +bFGF组 (ischemia+bFGFgroup)。结扎大鼠左冠状动脉复制缺血性心肌损伤模型。术前注入bFGF或生理盐水 ,于结扎后 2h、4h、8h观察大鼠缺血区心肌细胞内Bcl- 2、Bax的表达和血清SOD、心肌酶活性变化。结果 :(1)在急性心肌缺血 2h、4h时 ,缺血 +bFGF组心肌细胞Bcl- 2蛋白表达明显高于缺血 +生理盐水组 (P <0 0 1) ,而Bax蛋白表达显著低于缺血 +生理盐水组 (P <0 0 5 )。 (2 )在心肌缺血 4h ,缺血 +bFGF组血清SOD活性明显高于缺血 +生理盐水组 (P <0 0 1)。 (3)缺血 +bFGF组LDH、CK -MB、α -HBDH含量明显低于缺血 +生理盐水组 (P <0 0 5 )。结论AIM: To study the protective effects of basic fibroblast growth factor (bFGF) on myocardial ischemia in rats and their underlying mechanism. METHODS: A rat myocardial ischemic injury model was established by left coronary artery ligation. The rats were killed at 2 h, 4 h, 8 h after coronary artery occlusion. The samples of blood and myocardium were collected for observing the expression of Bcl-2 and Bax in myocardial cells and the changes of superoxide dismutase (SOD) or myocardial enzymes. RESULTS: The amount of Bcl-2 protein expression of myocardial cells in ischemia + bFGF group was significantly higher than that in ischemia+saline group (P<0.01) at 2 h, 4 h after coronary artery occlusion. However, the change of Bax protein expression was reversed (P<0.05). The activity of SOD in ischemia+bFGF group was higher than that in ischemia+saline group, and the changes of LDH, CK-MB and α-HBDH in ischemia+bFGF group were reversed (P<0.05) in serum. CONCLUSION: bFGF has protective effects against myocardial ischemia in rats. [

关 键 词：成纤维细胞生长因子2 心肌缺血 肌酸激酶 BCL-2 

分 类 号：R363[医药卫生—病理学]