^(131)I标记的抗肝癌血管内皮细胞单克隆抗体对肝癌治疗的实验研究  

Targeting^(131)I labeled monoclonal antibody against vascular endothelial cells for treatment of hepatocarcinoma

作　　者：夏红天[1] 黄志强[1] 袁玫[1] 于占洋[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《解放军医学杂志》2004年第11期986-988,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的　运用13 1I标记的抗肝癌血管内皮细胞单克隆抗体 ,研究靶向血管内皮细胞治疗肝癌的可行性。方法　建立裸小鼠人肝细胞癌动物模型 ,取 30只裸鼠随机分为 3组 ,分别为实验A组、实验B组和对照组 ,每组各 10只。实验A组在接种肝癌细胞的同时 ,经腹腔注射抗肝癌血管内皮细胞的单克隆抗体 ,每只 2 0 0 μg/ 2 0 0 μl,2次 /周 ;实验B组在接种肝癌细胞的同时 ,经腹腔注射同剂量的13 1I标记的抗肝癌血管内皮细胞的单克隆抗体 ;对照组在接种肝癌细胞的同时 ,经腹腔注射等量的生理盐水。观察肿瘤的生长情况 ,计算肿瘤的体积 ,计算抑瘤率。结果　实验A组的抑瘤率为 74 5 5 % ,实验B组为 86 36 % ;实验A、B组与对照组比较肿瘤明显受抑 (P <0 0 5 )。实验B组与实验A组比较显示了明显的放疗作用(P <0 0 5 )。HE及免疫组化染色观察证实 ,经单抗治疗后肿瘤区微血管内血栓形成 ,血管内皮细胞变性、坏死 ,血管周围大片肿瘤细胞坏死 ,瘤内血管密度明显降低。结论　抗肝癌血管内皮细胞单克隆抗体在动物实验中有明显的抑瘤作用 ,以此抗体为载体与核素相结合 ,可明显提高治疗肿瘤的疗效。Objective To study the possibility of targeting endothelial cells with the 131I labeled monoclonal antibody (mAb) for the treatment of hepatocarcinoma. Methods An animal model of human hepatocarcinoma in nude mice was reproduced, and tumor inhibiting activity of the 131I labeled mAb was tested. 30 nude mice with hepatocarcinoma were randomly divided into 3 groups: in group A the mice were treated with mAb 200μg/200μl twice a week; group B mice were treated with the 131I labeled mAb 200μg/200μl twice a week; control group mice were given normal saline in equal volume. Tumor growth in mice was observed. After the mice were sacrificed, the tumor was histologically examined and the intra-tumor microvessel density (TMVD) recorded. Results The tumor growth inhibition effect in mice treated with mAb was 74.55%. This effect was enhanced when treated with the 131I labeled mAb, as evidenced by an increase of tumor growth inhibition rate to 86.36%. Pathologically, Massive necrosis of tumor cells around the degenerated vessels was observed in the mAb treated mice. TMVD was significantly lower in the mAb treated mice than that in the untreated mice (P<0.05). Conclusion The mAb against vascular endothelial cells was effective in inhibiting the growth of hepatocarcinoma, and the 131I labeled mAb presented higher inhibitory effect on the tumor in an animal model.

关 键 词：癌 肝细胞 抗体 单克隆 血管内皮细胞 导向治疗 

分 类 号：R735.7[医药卫生—肿瘤]