荧光原位杂交检测垂体瘤染色体的异常  

Chromosomal abnormalities of pituitary adenomas detected by fluorescence in situ hybridization

作　　者：王清[1] 鲁晓杰[1] 蔺玉昌[1] 苗增利[1] 季卫阳[1] 李兵[1] 殷力维[1] 徐幸[1] 缪亦峰[1] 

机构地区：[1]南京医科大学附属无锡市第二人民医院神经外科,无锡214002

出　　处：《江苏医药》2004年第11期807-808,i002,共3页Jiangsu Medical Journal

基　　金：江苏省教育厅基金资助项目 ( 0 2KJD2 2 0 0 17);无锡市社会发展基金资助项目 (CS0 3 0 0 0 4)

摘　　要：目的　研究垂体瘤间期细胞遗传性畸变 ,探讨其异常与垂体瘤发生和发展的关系。方法　应用荧光原位杂交技术研究了 5 0例垂体瘤间期细胞核 7、8、11染色体数目的异常。结果　6 4 % (32 / 5 0 )的垂体瘤出现一个以上染色体数量的变异 ,其中以 11染色体的异常最为常见 2 8% (14 /5 0 ) ,其余依次为 7染色体 2 2 % (11/ 5 0 )、8染色体 2 0 % (10 / 5 0 )。 7、8染色体的异常与垂体瘤的临床病理特征未见明显相关 ,但 11号单体与垂体瘤的侵袭性密切相关。结论　垂体瘤 7、8、11染色体变异是频繁发生的。 7、8染色体的获得可能参与了肿瘤形成的起始阶段 ,而 11号染色体的丢失可能与肿瘤的演进及生物行为有关。Objective To study chromosome numerical aberrations and the relationship of the molecular cytogenetic alterations with the evolution and progression in pituitary adenoma.Methods Fluorescence in situ hybridization(FISH)was performed using centromeric probes of chromosome 7,8 and 11 to detect chromosome numerical aberrations in 50 pituitary adenomas.Results Of these 50 pituitary adenomas,32(64%)had an abnormal dosage of one or more of the 4 chromosomes tested,and the chromosome with the highest frequency of abnormal karyotypes revealed by FISH was 11(28%),followed by 7(22%)and 8(20%).There were no significant differences in the FISH results for the individual clinical subtypes of pituitary tumors,but the losses of chromosome 11 had marked correlations with the invasiveness of pituitary adenomas.Conclusion The chromosome 7,8 and 11 alterations are frequent in pituitary adenomas.These results reveal a nonrandom pattern of chromosomal alterations in pituitary adenomas,in particular gains of entire chromosome 7,8 and losses of chromosome 11 may contribute to the initiation or progression of pituitary adenomas.

关 键 词：垂体瘤 染色体数目 间期细胞 肿瘤形成 荧光原位杂交 异常 常见 性畸变 染色体变异 数量 

分 类 号：R736.4[医药卫生—肿瘤] R733[医药卫生—临床医学]