永生化人成牙本质细胞样细胞系体外矿化的初步观察  

Characterize the mineralization of human odontoblast-like cell line in vitro

作　　者：王捍国[1] 肖明振[1] 赵守亮[1] 郝建军[1] 郝玉庆[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2004年第11期612-614,共3页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目 (3 9970 792 ;3 0 2 70 3 74) ;国家教育部回国人员启动基金资助项目(2 0 0 0HG0 0 3 ) ;第四军医大学学术新人资助基金资助项目 (2 0 0 3年 )

摘　　要：目的 :探讨永生化人成牙本质细胞样细胞系hTERT -hOd -l的体外矿化特征。方法 :在矿化液连续培养细胞 5周 ,采用倒置显微镜、透射电镜进行组织学观察以及VonKossa染色。结果 :细胞在矿化液中生长良好 ,形成细胞结节 ,分泌细胞外基质 ,其中有平行排列的胶原纤维和针状晶体沉积。VonKossa染色显示细胞结节已发生矿化。结论 :hTERT -hOd -l细胞在体外培养条件下具有矿化能力。AIM:To charecterize the mineralization of huma n odontoblast-like cell line hTERT-hOd-l in vitro.METHODS: After being cultured in mineralizing medium for 5 weeks,the cells were observedby phase-contrast and transmission electron microscope (TEM),and stained by VonKossa.RESULTS:The cells grew well in mineralizing medium,and b ecame multilayered to form well-defined cell nodules gradually.Mineralization ofthem was confirmed histologically by Von Kossa staining.TEM revealed the presen ce of needle-shaped crystals associated with a network of collagen fibril in thesecreted matrix.CONCLUSION:hTERT-hOd-l has the ability of min eralization in vitro. [

关 键 词：矿化 细胞系 成牙本质细胞 细胞培养 

分 类 号：R780.2[医药卫生—口腔医学]