环胞素A和粉防己碱对牛脑微血管内皮细胞Rh-123摄取的影响  

Effect of Cyclosporin A and Tetrandrine on rhodamine 123 accumulationin in bovine brain capillary endothelial cells

作　　者：包金风[1] 刘国卿[2] 徐群渊[1] 

机构地区：[1]首都医科大学北京神经科学研究所,北京100054 [2]中国药科大学药理学教研室,江苏南京210009

出　　处：《中国药理学通报》2004年第11期1265-1268,共4页Chinese Pharmacological Bulletin

摘　　要：目的　研究环胞素A和粉防己碱对脑毛细血管内皮细胞上P 糖蛋白 (P glycoprotein ,P gp)的外排转运系统的作用。方法　利用荧光分光光度法研究环胞素A和粉防己碱对体外培养牛脑毛细血管内皮细胞罗丹明 12 3摄取的影响。结果　当给予P gp抑制剂环胞素A后 ,Rh 12 3在牛脑毛细血管内皮细胞 (braincapillaryendothelialcell,BCEC)单层的积累量明显增加。粉防己碱能够明显增加BCEC的Rh 12 3积累 ,并且呈剂量依赖性。结论　研究表明 ,此种方法是一个敏感 ,可靠的研究方法 ;粉防己碱是一个很有前途的血脑屏障上PAim To study the effect of cyclosporin A and tetrand ri ne on P-glycoprotein (P-gp)of bovine brain capillary endothelial cell. M ethods The fluorescent dye, rhodamine-123 (Rh-123) was used to evaluate t he functional activity of the P-glycoprotein (P-gp) efflux transport system in primary cultured bovine brain capillary endothelial cell (BCEC) monolayer. Results Rhodamine-123 accumulation was increased significantly in monola yer treated with the P-gp modifying agent, cyclosporin A and tetrandrine. Conclusion The observation suggests that this Rh-123 method is sens itive, stable to evaluate the function of P-gp of blood-brain barrier (BBB). R h-123 accumulation is also increased by tetrandrine in dose-dependent manner.

关 键 词：P-糖蛋白 脑毛细血管内皮细胞 血脑屏障 罗丹明-123 环胞素A 粉防己碱 

分 类 号：R-332[医药卫生] R284.1